1887

Abstract

A novel actinomycete strain, designated CS5-AC15, was isolated from a soil sample collected from a cliff on Mara Island, Jeju, Republic of Korea, and subjected to a polyphasic taxonomic analysis. The isolate produced well-developed, yellow substrate mycelium and white aerial mycelium that differentiated into straight or flexuous chains of smooth-surfaced spores. 16S rRNA gene sequence analyses showed that the organism belonged to the family and formed a tight cluster with the type strain of (97.4 % sequence similarity). Chemotaxonomic characteristics were consistent with its assignment to the genus in that the isolate had -diaminopimelic acid as the diagnostic diamino acid in the cell wall, madurose as the characteristic sugar, -acetyl type of murein in the peptidoglycan, MK-9(H) and MK-9(H) as major menaquinones and a polar lipid profile containing diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and unknown phospholipids. Mycolic acids were not detected. The predominant fatty acids were C, Cω9 and iso-C. The DNA G+C content was 70.9 mol%. DNA relatedness of strain CS5-AC15 and JCM 10648 was 37.9±0.7 %. On the basis of the phenotypic, phylogenetic and DNA–DNA hybridization data, strain CS5-AC15 is assigned to a novel species of the genus , for which the name sp. nov. is proposed (type strain CS5-AC15  = KCTC 19559  = DSM 45251).

Funding
This study was supported by the:
  • 21C Frontier Program of Microbial Genomics and Applications (Award 11-2008-01-003-00)
  • Ministry of Education, Science & Technology (MEST) of the Republic of Korea
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2012-04-01
2021-10-26
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