1887

Abstract

A Gram-negative, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated MA1-1, was isolated from a sea squirt () collected from the South Sea, Korea. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain MA1-1 is phylogenetically closely related to species and to . It exhibited 16S rRNA gene sequence similarities of 97.3, 97.1 and 97.3 % to the type strains of , and , respectively. Strain MA1-1 contained Q-10 as the predominant ubiquinone and Cω7 as the predominant fatty acid. The polar lipid profile of strain MA1-1 was similar to those of the type strains of and . was found to be phylogenetically and chemotaxonomically more closely related to species and strain MA1-1 than to , the type species of the genus . The DNA G+C content of strain MA1-1 was 57.9 mol%, and DNA–DNA relatedness to the type strains of the two species and was 9–14 %. Differential phenotypic properties, together with the observed phylogenetic and genetic distinctiveness, distinguished strain MA1-1 from the two species and . On the basis of the data presented, strain MA1-1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MA1-1 ( = KCTC 23699  = CCUG 61486). In this study, it is also proposed that is reclassified as a member of the genus , comb. nov. (type strain GA2-M15  = DSM 19593  = KACC 12675). An emended description of the genus is also presented.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.035113-0
2012-08-01
2019-12-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/8/1825.html?itemId=/content/journal/ijsem/10.1099/ijs.0.035113-0&mimeType=html&fmt=ahah

References

  1. Bruns A., Rohde M., Berthe-Corti L.. ( 2001;). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51:, 1997–2006. [CrossRef][PubMed]
    [Google Scholar]
  2. Cowan S. T., Steel K. J.. ( 1965;). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  4. Ivanova E. P., Webb H., Christen R., Zhukova N. V., Kurilenko V. V., Kalinovskaya N. I., Crawford R. J.. ( 2010;). Celeribacter neptunius gen. nov., sp. nov., a new member of the class Alphaproteobacteria. . Int J Syst Evol Microbiol 60:, 1620–1625. [CrossRef][PubMed]
    [Google Scholar]
  5. Kim B.-Y., Weon H.-Y., Son J.-A., Lee C.-M., Hong S.-B., Jeon Y.-A., Koo B.-S., Kwon S.-W.. ( 2009;). Thalassobacter arenae sp. nov., isolated from sea sand in Korea. . Int J Syst Evol Microbiol 59:, 487–490. [CrossRef][PubMed]
    [Google Scholar]
  6. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  7. Lányí B.. ( 1987;). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  8. Leifson E.. ( 1963;). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85:, 1183–1184.[PubMed]
    [Google Scholar]
  9. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  10. Ostle A. G., Holt J. G.. ( 1982;). Nile blue A as a fluorescent stain for poly-β-hydroxybutyrate. . Appl Environ Microbiol 44:, 238–241.[PubMed]
    [Google Scholar]
  11. Pujalte M. J., Macián M. C., Arahal D. R., Garay E.. ( 2005;). Thalassobacter stenotrophicus Macián et al. 2005 is a later synonym of Jannaschia cystaugens Adachi et al. 2004, with emended description of the genus Thalassobacter. . Int J Syst Evol Microbiol 55:, 1959–1963. [CrossRef][PubMed]
    [Google Scholar]
  12. Romanenko L. A., Tanaka N., Frolova G. M., Svetashev V. I., Mikhailov V. V.. ( 2011;). Litoreibacter albidus gen. nov., sp. nov. and Litoreibacter janthinus sp. nov., members of the class Alphaproteobacteria isolated from the seashore. . Int J Syst Evol Microbiol 61:, 148–154. [CrossRef][PubMed]
    [Google Scholar]
  13. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note no. 101. . Newark, DE:: MIDI, Inc.;
  14. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  15. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  16. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  17. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H.. ( 1996;). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46:, 502–505. [CrossRef]
    [Google Scholar]
  18. Yoon J.-H., Lee S. T., Park Y.-H.. ( 1998;). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48:, 187–194. [CrossRef][PubMed]
    [Google Scholar]
  19. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H.. ( 2003;). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. . Int J Syst Evol Microbiol 53:, 53–57. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.035113-0
Loading
/content/journal/ijsem/10.1099/ijs.0.035113-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error