1887

Abstract

Strains of Gram-negative, rod-shaped, non-spore-forming bacteria were isolated from nitrogen-fixing nodules of the native legumes (from Zambia) and (from Texas, USA). Phylogenetic analysis of the 16S rRNA gene showed that the novel strains belong to the genus , with ≥96.1 % sequence similarity with type strains of this genus. The closest relative of the representative strains Lut6 and WSM3557 was TFB, with 97.6–98.0 % similarity, while WSM3693 was most closely related to 5420S-16, with 98.8 % similarity. Analysis of the concatenated sequences of four housekeeping gene loci (, , and ) and cellular fatty acid profiles confirmed the placement of Lut6, WSM3557 and WSM3693 within the genus . DNA–DNA relatedness values, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of Lut6, WSM3557 and WSM3693 from each other and from other species with validly published names. The sequence of Lut6 was placed in a clade that contained strains of , and , while the 100 % identical sequences of WSM3557 and WSM3693 clustered with , and strains. Concatenated sequences for and show that the sequences of Lut6, WSM3557 and WSM3693 were most closely related to that of CFN42 . On the basis of genotypic, phenotypic and DNA relatedness data, three novel species of are proposed: sp. nov. (type strain Lut6  = LMG 26460  = HAMBI 3236), sp. nov. (type strain WSM3557  = LMG 26455  = HAMBI 3237) and sp. nov. (type strain WSM3693  = LMG 26454  = HAMBI 3238).

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2012-11-01
2019-09-18
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