1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_1

sp. nov., a haloalkaliphilic actinobacterium isolated from a salt pan Free

Abstract

A Gram-positive, non-motile, non-spore-forming actinobacterium designated strain Sua-BAC020 was isolated from brine from Sua salt pan in Botswana. The strain was alkaliphilic and moderately halophilic, displaying optimal growth at 35–37 °C, pH 9 and 2.5 % (w/v) NaCl. Comparative 16S rRNA gene sequence analysis showed that strain Sua-BAC020 belonged to the genus , sharing 96.2–99.0 % sequence similarity with the type strains of recognized species within this genus. DNA–DNA hybridization with the type strains of species that showed the closest phylogenetic affiliation, (16S rRNA gene sequence similarity, 98.9 %), (99.0 %), (97.5 %), (97.4 %) and (97.2 %), gave relatedness values of 10–45 %. The peptidoglycan type of strain Sua-BAC020 was A4α, -Lys–Gly–-Asp. Cells of the isolate contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and unidentified glycolipids as major polar lipids, MK-8, MK-9 and MK-7 were the predominant menaquinones, and the major fatty acids (>10 %) were anteiso-C and anteiso-C. The DNA G+C content of strain Sua-BAC020 was 64.8 mol%. Based on DNA–DNA hybridization, and physiological and biochemical tests, strain Sua-BAC020 is distinct from all recognized species, suggesting that this strain represents a novel species, for which the name sp. nov. is proposed. The type strain is Sua-BAC020 ( = DSM 22748 = NCCB 100309).

  • Published Online:
Funding
This study was supported by the:
  • National Research Foundation of South Africa
© 2013 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.035006-0
2013-01-01
2023-09-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/1/41.html?itemId=/content/journal/ijsem/10.1099/ijs.0.035006-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Baker G. C., Smith J. J., Cowan D. A. ( 2003 ). Review and re-analysis of domain-specific 16S primers. . J Microbiol Methods 55, 541–555. [View Article] [PubMed]
    [Google Scholar]
  3. Bligh E. G., Dyer W. J. ( 1959 ). A rapid method of total lipid extraction and purification. . Can J Biochem Physiol 37, 911–917. [View Article] [PubMed]
    [Google Scholar]
  4. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. ( 1977 ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81, 461–466. [View Article] [PubMed]
    [Google Scholar]
  5. CLSI ( 2006 ). Document M2–A9 and M7–A7, Performance Standards for Antimicrobial Susceptibility Testing; 16th Supplement. . Wayne, PA:: Clinical and Laboratory Standards Institute;
  6. Collins M. D., Jones D. ( 1980 ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. . J Appl Bacteriol 48, 459–470. [View Article]
    [Google Scholar]
  7. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221–230. [View Article] [PubMed]
    [Google Scholar]
  8. Collins M. D., Lawson P. A., Labrenz M., Tindall B. J., Weiss N., Hirsch P. ( 2002 ). Nesterenkonia lacusekhoensis sp. nov., isolated from hypersaline Ekho Lake, East Antarctica, and emended description of the genus Nesterenkonia . . Int J Syst Evol Microbiol 52, 1145–1150. [View Article] [PubMed]
    [Google Scholar]
  9. Cowan S. T., Steel K. J. ( 1965 ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
     [Google Scholar]
  10. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133–142. [PubMed] [CrossRef]
    [Google Scholar]
  11. Delgado O., Quillaguamán J., Bakhtiar S., Mattiasson B., Gessesse A., Hatti-Kaul R. ( 2006 ). Nesterenkonia aethiopica sp. nov., an alkaliphilic, moderate halophile isolated from an Ethiopian soda lake. . Int J Syst Evol Microbiol 56, 1229–1232. [View Article] [PubMed]
    [Google Scholar]
  12. Dyall-Smith M. ( 2000 ). Halohandbook: Protocols for haloarchaeal genetics. Version 3. http://www.haloarchaea.com/resources/halohandbook
  13. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [PubMed] [CrossRef]
    [Google Scholar]
  14. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  15. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406–416. [View Article]
    [Google Scholar]
  16. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 95–98.
     [Google Scholar]
  17. Huss V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184–192. [View Article]
    [Google Scholar]
  18. Kimura M. ( 1983 ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  19. Klatte S., Kroppenstedt R. M., Rainey F. A. ( 1994 ). Rhodococcus opacus sp. nov., an unusual nutritionally versatile Rhodococcus-species. . Syst Appl Microbiol 17, 355–360. [CrossRef]
    [Google Scholar]
  20. Kuykendall L. D., Roy M. A., O’Neill J. J., Devine T. E. ( 1988 ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradorhizobium japonicum . . Int J Syst Bacteriol 38, 358–361. [View Article]
    [Google Scholar]
  21. Lee J.-H., Rho J. B., Park K.-J., Kim C. B., Han Y.-S., Choi S. H., Lee K.-H., Park S.-J. ( 2004 ). Role of flagellum and motility in pathogenesis of Vibrio vulnificus . . Infect Immun 72, 4905–4910. [View Article] [PubMed]
    [Google Scholar]
  22. Leifson E. ( 1963 ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85, 1183–1184.[PubMed]
    [Google Scholar]
  23. Li W.-J., Chen H.-H., Zhang Y.-Q., Schumann P., Stackebrandt E., Xu L.-H., Jiang C.-L. ( 2004 ). Nesterenkonia halotolerans sp. nov. and Nesterenkonia xinjiangensis sp. nov., actinobacteria from saline soils in the west of China. . Int J Syst Evol Microbiol 54, 837–841. [PubMed] [CrossRef]
    [Google Scholar]
  24. Li W.-J., Chen H.-H., Kim C.-J., Zhang Y.-Q., Park D.-J., Lee J.-C., Xu L.-H., Jiang C.-L. ( 2005 ). Nesterenkonia sandarakina sp. nov. and Nesterenkonia lutea sp. nov., novel actinobacteria, and emended description of the genus Nesterenkonia . . Int J Syst Evol Microbiol 55, 463–466. [PubMed] [CrossRef]
    [Google Scholar]
  25. Li W.-J., Zhang Y.-Q., Schumann P., Liu H.-Y., Yu L.-Y., Zhang Y.-Q., Stackebrandt E., Xu L.-H., Jiang C.-L. ( 2008 ). Nesterenkonia halophila sp. nov., a moderately halophilic, alkalitolerant actinobacterium isolated from a saline soil. . Int J Syst Evol Microbiol 58, 1359–1363. [View Article] [PubMed]
    [Google Scholar]
  26. Luo H.-Y., Miao L.-H., Fang C., Yang P.-L., Wang Y.-R., Shi P.-J., Yao B., Fan Y.-L. ( 2008 ). Nesterenkonia flava sp. nov., isolated from paper-mill effluent. . Int J Syst Evol Microbiol 58, 1927–1930. [View Article] [PubMed]
    [Google Scholar]
  27. Luo H.-Y., Wang Y.-R., Miao L.-H., Yang P. L., Shi P.-J., Fang C.-X., Yao B., Fan Y.-L. ( 2009 ). Nesterenkonia alba sp. nov., an alkaliphilic actinobacterium isolated from the black liquor treatment system of a cotton pulp mill. . Int J Syst Evol Microbiol 59, 863–868. [View Article] [PubMed]
    [Google Scholar]
  28. Mesbah M., Premachandran U., Whitman W. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39, 159–167. [View Article]
    [Google Scholar]
  29. Miller L. T. ( 1982 ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16, 584–586.[PubMed]
    [Google Scholar]
  30. Minnikin D. E., Alshamaony L., Goodfellow M. ( 1975 ). Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. . J Gen Microbiol 88, 200–204. [View Article] [PubMed]
    [Google Scholar]
  31. Mota R. R., Márquez M. C., Arahal D. R., Mellado E., Ventosa A. ( 1997 ). Polyphasic taxonomy of Nesterenkonia halobia . . Int J Syst Bacteriol 47, 1231–1235. [PubMed] [CrossRef]
    [Google Scholar]
  32. Rhuland L. E., Work E., Denman R. F., Hoare D. S. ( 1955 ). The behaviour of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. . J Am Chem Soc 77, 4844–4846. [View Article]
    [Google Scholar]
  33. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  34. Schleifer K. H. ( 1985 ). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18, 123–156. [View Article]
    [Google Scholar]
  35. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407–477.[PubMed]
    [Google Scholar]
  36. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846–849. [View Article]
    [Google Scholar]
  37. Stackebrandt E., Koch C., Gvozdiak O., Schumann P. ( 1995 ). Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. . Int J Syst Bacteriol 45, 682–692. [View Article] [PubMed]
    [Google Scholar]
  38. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [PubMed] [CrossRef]
    [Google Scholar]
  39. Teather R. M., Wood P. J. ( 1982 ). Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. . Appl Environ Microbiol 43, 777–780.[PubMed]
    [Google Scholar]
  40. Tindall B. J., Sikorski J., Smibert R. M., Kreig N. R. ( 2007 ). Phenotyoic characterization and the principles of comparative systematic. . In Methods for General and Molecular Microbiology, , 3rd edn., pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  41. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  42. Yoon J.-H., Jung S.-Y., Kim W., Nam S.-W., Oh T.-K. ( 2006 ). Nesterenkonia jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 56, 2587–2592. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.035006-0
Loading
Nesterenkonia suensis sp. nov., a haloalkaliphilic actinobacterium isolated from a salt pan
Int J Syst Evol Microbiol 63, 41 (2013); https://doi.org/10.1099/ijs.0.035006-0
/content/journal/ijsem/10.1099/ijs.0.035006-0
/content/journal/ijsem/10.1099/ijs.0.035006-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error