1887

Abstract

A Gram-positive, non-motile, non-spore-forming actinobacterium designated strain Sua-BAC020 was isolated from brine from Sua salt pan in Botswana. The strain was alkaliphilic and moderately halophilic, displaying optimal growth at 35–37 °C, pH 9 and 2.5 % (w/v) NaCl. Comparative 16S rRNA gene sequence analysis showed that strain Sua-BAC020 belonged to the genus , sharing 96.2–99.0 % sequence similarity with the type strains of recognized species within this genus. DNA–DNA hybridization with the type strains of species that showed the closest phylogenetic affiliation, (16S rRNA gene sequence similarity, 98.9 %), (99.0 %), (97.5 %), (97.4 %) and (97.2 %), gave relatedness values of 10–45 %. The peptidoglycan type of strain Sua-BAC020 was A4α, -Lys–Gly–-Asp. Cells of the isolate contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and unidentified glycolipids as major polar lipids, MK-8, MK-9 and MK-7 were the predominant menaquinones, and the major fatty acids (>10 %) were anteiso-C and anteiso-C. The DNA G+C content of strain Sua-BAC020 was 64.8 mol%. Based on DNA–DNA hybridization, and physiological and biochemical tests, strain Sua-BAC020 is distinct from all recognized species, suggesting that this strain represents a novel species, for which the name sp. nov. is proposed. The type strain is Sua-BAC020 ( = DSM 22748 = NCCB 100309).

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2013-01-01
2020-01-28
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