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Volume 62, Issue Pt_8

sp. nov., isolated from coal mine soil Free

Abstract

A Gram-positive, aerobic, motile, rod-shaped bacterium, designated strain T26, was isolated from subsurface soil of Tianjin coal mine, China. Colonies were yellow–white, convex, circular, smooth and non-transparent on R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain T26 was closely related to members of the genus and a member of the genus with 96.8–94.7 % and 96.7 % gene sequence similarities, respectively. The peptidoglycan type of strain T26 was A4β, containing -ornithine–-glutamic acid as the interpeptide bridge. The cell-wall sugars were rhamnose, galactose, xylose and inositol. The major fatty acids (>10 %) were anteiso-C (33.6 %), anteiso-C A (22.1 %), C (14.4 %) and C (12.1 %). The predominant respiratory quinone was MK-9(H) and the genomic DNA G+C content was 74.4 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol-mannosides and phosphatidylinositol. Comparison of phenotypic and phylogenetic characteristics between strain T26 and related organisms revealed that the new isolate represented a novel species of the genus , for which the name sp. nov. is proposed. The type strain is T26 ( = CGMCC 1.10786 = KCTC 19824 = CCTCC AB2010450).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31010103903)
© 2012 IUMS
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2012-08-01
2023-09-27
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Cellulomonas carbonis sp. nov., isolated from coal mine soil
Int J Syst Evol Microbiol 62, 2004 (2012); https://doi.org/10.1099/ijs.0.034934-0
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