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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 62, Issue Pt_8

sp. nov., isolated from coal mine soil Free

Abstract

A Gram-positive, aerobic, motile, rod-shaped bacterium, designated strain T26, was isolated from subsurface soil of Tianjin coal mine, China. Colonies were yellow–white, convex, circular, smooth and non-transparent on R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain T26 was closely related to members of the genus and a member of the genus with 96.8–94.7 % and 96.7 % gene sequence similarities, respectively. The peptidoglycan type of strain T26 was A4β, containing -ornithine–-glutamic acid as the interpeptide bridge. The cell-wall sugars were rhamnose, galactose, xylose and inositol. The major fatty acids (>10 %) were anteiso-C (33.6 %), anteiso-C A (22.1 %), C (14.4 %) and C (12.1 %). The predominant respiratory quinone was MK-9(H) and the genomic DNA G+C content was 74.4 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol-mannosides and phosphatidylinositol. Comparison of phenotypic and phylogenetic characteristics between strain T26 and related organisms revealed that the new isolate represented a novel species of the genus , for which the name sp. nov. is proposed. The type strain is T26 ( = CGMCC 1.10786 = KCTC 19824 = CCTCC AB2010450).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31010103903)
© 2012 IUMS
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2012-08-01
2025-04-18
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References

  1. An D.-S., Im W.-T., Yang H.-C., Kang M. S., Kim K. K., Jin L., Kim M. K., Lee S.-T. 2005; Cellulomonas terrae sp. nov., a cellulolytic and xylanolytic bacterium isolated from soil. Int J Syst Evol Microbiol 55:1705–1709 [View Article][PubMed]
     [Google Scholar]
  2. Bagnara C., Toci R., Gaudin C., Belaich J. P. 1985; Isolation and characterization of a cellulolytic microorganism, Cellulomonas fermentans sp. nov.. Int J Syst Bacteriol 35:502–507 [View Article]
     [Google Scholar]
  3. Bergey D. H., Harrison F. C., Breed R. S., Hammer B. W., Huntoon F. M. (editors) 1923 Bergey’s Manual of Determinative Bacteriology Baltimore, MD: Williams and Wilkins;
     [Google Scholar]
  4. Brown J. M., Frazier R. P., Morey R. E., Steigerwalt A. G., Pellegrini G. J., Daneshvar M. I., Hollis D. G., McNeil M. M. 2005; Phenotypic and genetic characterization of clinical isolates of CDC coryneform group A-3: proposal of a new species of Cellulomonas, Cellulomonas denverensis sp. nov.. J Clin Microbiol 43:1732–1737 [View Article][PubMed]
     [Google Scholar]
  5. Cappuccino J. G., Sherman N. 2002 Microbiology: a Laboratory Manual, 6th edn. Menlo Park, CA: Benjamin/Cummings;
     [Google Scholar]
  6. Clark F. E. 1952; The generic classification of the soil corynebacteria. Int Bull Bacteriol NomTax 2:45–56 [View Article]
     [Google Scholar]
  7. Collins M. D., Pascual C. 2000; Reclassification of Actinomyces humiferus (Gledhill and Casida) as Cellulomonas humilata nom. corrig., comb. nov.. Int J Syst Evol Microbiol 50:661–663 [View Article][PubMed]
     [Google Scholar]
  8. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria Cambridge, UK: Cambridge University Press;
     [Google Scholar]
  9. Dussault H. P. 1955; An improved technique for staining red halophilic bacteria. J Bacteriol 70:484–485[PubMed]
     [Google Scholar]
  10. Elberson M. A., Malekzadeh F., Yazdi M. T., Kameranpour N., Noori-Daloii M. R., Matte M. H., Shahamat M., Colwell R. R., Sowers K. R. 2000; Cellulomonas persica sp. nov. and Cellulomonas iranensis sp. nov., mesophilic cellulose-degrading bacteria isolated from forest soils. Int J Syst Evol Microbiol 50:993–996 [View Article][PubMed]
     [Google Scholar]
  11. Fan H., Su C., Wang Y., Yao J., Zhao K., Wang Y., Wang G. 2008; Sedimentary arsenite-oxidizing and arsenate-reducing bacteria associated with high arsenic groundwater from Shanyin, Northwestern China. J Appl Microbiol 105:529–539 [View Article][PubMed]
     [Google Scholar]
  12. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
     [Google Scholar]
  13. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
     [Google Scholar]
  14. Funke G., Ramos C. P., Collins M. D. 1995; Identification of some clinical strains of CDC coryneform group A-3 and A-4 bacteria as Cellulomonas species and proposal of Cellulomonas hominis sp. nov. for some group A-3 strains. J Clin Microbiol 33:2091–2097[PubMed]
     [Google Scholar]
  15. Guindon S., Lethiec F., Duroux P., Gascuel O. 2005; PHYML Online–a web server for fast maximum likelihood-based phylogenetic inference. Nucleic Acids Res 33:Web Server issueW557–W559 [View Article][PubMed]
     [Google Scholar]
  16. Hinz K. H., Ryll M., Köhler B. 1998; Detection of acid production from carbohydrates by Riemerella anatipestifer and related organisms using the buffered single substrate test. Vet Microbiol 60:277–284 [View Article][PubMed]
     [Google Scholar]
  17. Jones B. E., Grant W. D., Duckworth A. W., Schumann P., Weiss N., Stackebrandt E. 2005; Cellulomonas bogoriensis sp. nov., an alkaliphilic cellulomonad. Int J Syst Evol Microbiol 55:1711–1714 [View Article][PubMed]
     [Google Scholar]
  18. Kang M. S., Im W. T., Jung H. M., Kim M. K., Goodfellow M., Kim K. K., Yang H. C., An D. S., Lee S. T. 2007; Cellulomonas composti sp. nov., a cellulolytic bacterium isolated from cattle farm compost. Int J Syst Evol Microbiol 57:1256–1260 [View Article][PubMed]
     [Google Scholar]
  19. Khan S. T., Harayama S., Tamura T., Ando K., Takagi M., Kazuo S. Y. 2009; Paraoerskovia marina gen. nov., sp. nov., an actinobacterium isolated from marine sediment. Int J Syst Evol Microbiol 59:2094–2098 [View Article][PubMed]
     [Google Scholar]
  20. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (SAB Technical Series no. 20) pp. 173–199 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
     [Google Scholar]
  21. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
     [Google Scholar]
  22. Lee C. M., Weon H. Y., Hong S. B., Jeon Y. A., Schumann P., Kroppenstedt R. M., Kwon S. W., Stackebrandt E. 2008; Cellulomonas aerilata sp. nov., isolated from an air sample. Int J Syst Evol Microbiol 58:2925–2929 [View Article][PubMed]
     [Google Scholar]
  23. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
     [Google Scholar]
  24. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  25. Rhoades K. R., Rimler R. B., Sandhu T. S. 1989; Pasteurellosis and pseudotuberculosis. In A Laboratory Manual for the Isolation and Identification of Avian Pathogens, 3rd edn. pp. 14–21 Edited by Purchase H. G., Arp L. H., Domermuth C. H., Pearson J. E. Kennet Square, PA: American Association of Avian Pathologists;
     [Google Scholar]
  26. Rivas R., Trujillo M. E., Mateos P. F., Martínez-Molina E., Velázquez E. 2004; Cellulomonas xylanilytica sp. nov., a cellulolytic and xylanolytic bacterium isolated from a decayed elm tree. Int J Syst Evol Microbiol 54:533–536 [View Article][PubMed]
     [Google Scholar]
  27. Rusznyák A., Tóth E. M., Schumann P., Spröer C., Makk J., Szabó G., Vladár P., Márialigeti K., Borsodi A. K. 2011; Cellulomonas phragmiteti sp. nov., a cellulolytic bacterium isolated from reed (Phragmites australis) periphyton in a shallow soda pond. Int J Syst Evol Microbiol 61:1662–1666 [View Article][PubMed]
     [Google Scholar]
  28. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  29. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
  30. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477[PubMed]
     [Google Scholar]
  31. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  32. Stackebrandt E., Kandler O. 1979; Taxonomy of the genus Cellulomonas, based on phenotypic characters and deoxyribonucleic acid-deoxyribonucleic acid homology, and proposal of seven neotype strains. Int J Syst Bacteriol 29:273–282 [View Article]
     [Google Scholar]
  33. Stackebrandt E., Keddie R. M. 1986; Genus Cellulomonas . In Bergey’s Manual of Systematic Bacteriology vol. 2 pp. 1325–1329 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
     [Google Scholar]
  34. Stackebrandt E., Prauser H. 1991; The family Cellulomonadaceae . In The Prokaryotes pp. 1323–1345 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York, NY: Springer-Verlag;
     [Google Scholar]
  35. Stackebrandt E., Breymann S., Steiner U., Prauser H., Weiss N., Schumann P. 2002; Re-evaluation of the status of the genus Oerskovia, reclassification of Promicromonospora enterophila (Jáger et al. 1983) as Oerskovia enterophila comb. nov. and description of Oerskovia jenensis sp. nov. and Oerskovia paurometabola sp. nov.. Int J Syst Evol Microbiol 52:1105–1111 [View Article][PubMed]
     [Google Scholar]
  36. Stackebrandt E., Schumann P., Prauser H. 2006; The Family Cellulomonadaceae . In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn. pp. 983–1001 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
     [Google Scholar]
  37. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231[PubMed]
     [Google Scholar]
  38. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  39. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  40. Tindall B. J. 1990; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
     [Google Scholar]
  41. Weeger W., Lièvremont D., Perret M., Lagarde F., Hubert J. C., Leroy M., Lett M. C. 1999; Oxidation of arsenite to arsenate by a bacterium isolated from an aquatic environment. Biometals 12:141–149 [View Article][PubMed]
     [Google Scholar]
  42. Yi H., Schumann P., Chun J. 2007; Demequina aestuarii gen. nov., sp. nov., a novel actinomycete of the suborder Micrococcineae, and reclassification of Cellulomonas fermentans Bagnara et al. 1985 as Actinotalea fermentans gen. nov., comb. nov.. Int J Syst Evol Microbiol 57:151–156 [View Article][PubMed]
     [Google Scholar]
  43. Yoon M. H., Ten L. N., Im W. T., Lee S. T. 2008; Cellulomonas chitinilytica sp. nov., a chitinolytic bacterium isolated from cattle-farm compost. Int J Syst Evol Microbiol 58:1878–1884 [View Article][PubMed]
     [Google Scholar]
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Cellulomonas carbonis sp. nov., isolated from coal mine soil
Int J Syst Evol Microbiol 62, 2004 (2012); https://doi.org/10.1099/ijs.0.034934-0
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