1887

Abstract

A Gram-staining-negative, non-spore-forming, facultatively anaerobic, non-flagellated, non-gliding, rod-shaped bacterium, designated strain BR-18, was isolated from marine sand collected on the western coast of South Korea. The taxonomic position of the novel strain was determined using a polyphasic approach. Strain BR-18 grew optimally at 25 °C, at pH 6.5–7.0 and in the absence of NaCl. In phylogenetic analyses based on 16S rRNA gene sequences, the novel strain fell within a clade comprising members of the genus and appeared most closely related to BR-3 (96.6 % sequence similarity) and WPCB133 (95.9 %). The novel strain showed lower levels of 16S rRNA gene sequence similarity with the type strains of other members of the genus (93.4–95.5 %) and those of other species included in the phylogenetic analyses (<91.6 %). Strain BR-18 contained MK-7 as its predominant menaquinone, summed feature 3 (Cω7 and/or iso-C 2-OH) and iso-C as its major fatty acids, phosphatidylethanolamine and an unidentified aminophospholipid as its major polar lipids, and sphingolipids. The genomic DNA G+C content of the novel strain was 42.4 mol%. Based on the phylogenetic and phenotypic data, strain BR-18 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BR-18 ( = KCTC 23697  = CCUG 61484).

Funding
This study was supported by the:
  • , Ministry of Education, Science and Technology of South Korea , (Award 11-2008-00-002-00)
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2012-12-01
2020-10-23
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