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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 62, Issue Pt_10

Reclassification of Qiu 2009 and Qiu 2009 as comb. nov. and comb. nov. and emended description of the genus Free

Abstract

We have carried out a polyphasic taxonomic characterization of DSM 19037 and DSM 19038, which are closely related phylogenetically to LMG 24411. All three strains are Gram-stain-positive, non-motile, moderately halotolerant and non-spore-forming. 16S rRNA gene sequence analyses showed that the strains constituted a coherent cluster, with sequence similarities between 99.7 and 98.7 %. The percentage similarity on the basis of amino acid sequences deduced from partial gene nucleotide sequences of these three type strains was 96.1–92.7 %. Phylogenetic trees based on the 16S rRNA gene and GyrB amino acid sequences, obtained by using three different algorithms, were consistent and showed that these three species constituted a deeply rooted cluster separated from the clades represented by the genera , , , , , , and , supporting their placement in the genus . All three type strains have menaquinone MK-8 as the major respiratory quinone and showed similar fatty acid profiles. The main polar lipids present in the three type strains were diphosphatidylglycerol and phosphatidylglycerol, and the three strains showed peptidoglycan type A4α with -lysine as the diagnostic diamino acid. The DNA GC contents of DSM 19037, DSM 19038 and LMG 24411 were 53.1, 50.2 and 53.7 mol%, respectively. The level of DNA–DNA hybridization among the three strains was 57–39 %, indicating that they are members of different species of the genus . The phenotypic data are consistent with the placement of these three species in a single genus and support their differentiation at the species level. On the basis of these data, we have emended the description of the genus and propose the reclassification of and to the genus , as comb. nov. (type strain ge10  = DSM 19037  = CGMCC 1.6762) and comb. nov. (type strain ge14  = DSM 19038  = CGMCC 1.6763).

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Funding
This study was supported by the:
  • Indian Department of Biotechnology
© 2012 IUMS
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2012-10-01
2024-04-25
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Reclassification of Bacillus beijingensis Qiu et al. 2009 and Bacillus ginsengi Qiu et al. 2009 as Bhargavaea beijingensis comb. nov. and Bhargavaea ginsengi comb. nov. and emended description of the genus Bhargavaea
Int J Syst Evol Microbiol 62, 2495 (2012); https://doi.org/10.1099/ijs.0.034850-0
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