sp. nov., isolated from a microbial consortium on a cheese Free

Abstract

A novel halophilic, Gram-reaction-negative, strictly aerobic, non-motile, rod-shaped and oxidase- and catalase-positive bacterial strain, designated WCC 4520, was isolated from a semi-hard, Raclette-type cheese. The colonies were yellow–orange; flexirubin-type pigments were not found. The strain hydrolysed gelatin, hippurate, tyrosine and Tweens 20 and 80. Optimal growth was observed with 6–8 % (w/v) NaCl, at pH 7–8 and at 27–30 °C. The genomic DNA G+C content was 33.6 mol%. In phylogenetic analysis based on 16S rRNA gene sequences, strain WCC 4520 appeared to be a member of the family and the closest phylogenetic neighbours were identified as DSM 5423 (94.0 % 16S rRNA gene sequence similarity) and CCUG 56752 (94.0 %). The predominant cellular fatty acids were iso-C, anteiso-C, iso-C G and iso-C 3-OH. The only detected quinone was MK-6 and the major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and an unidentified polar lipid. Minor polar lipids and traces of polyamines were also detected. On the basis of the data presented, strain WCC 4520 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WCC 4520 ( = LMG 25857 = CCUG 59705).

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2012-08-01
2024-03-29
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References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. 1996; Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47:39–52 [View Article]
    [Google Scholar]
  2. Auling G., Busse H.-J., Pilz F., Webb L., Kneifel H., Claus D. 1991; Rapid differentiation, by polyamine analysis, of Xanthomonas strains from phytopathogenic pseudomonads and other members of the class Proteobacteria interacting with plants. Int J Syst Bacteriol 41:223–228 [View Article]
    [Google Scholar]
  3. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  4. Bochner B. R. 2009; Global phenotypic characterization of bacteria. FEMS Microbiol Rev 33:191–205 [View Article][PubMed]
    [Google Scholar]
  5. Bowman J. P., McCammon S. A., Lewis T., Skerratt J. H., Brown J. L., Nichols D. S., McMeekin T. A. 1998; Psychroflexus torquis gen. nov., sp. nov., a psychrophilic species from Antarctic sea ice, and reclassification of Flavobacterium gondwanense (Dobson et al. 1993) as Psychroflexus gondwanense gen. nov., comb. nov.. Microbiology 144:1601–1609 [View Article][PubMed]
    [Google Scholar]
  6. Buck J. D. 1982; Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993[PubMed]
    [Google Scholar]
  7. Buller N. B. (editor) 2004 Bacteria from Fish and Other Aquatic Animals: A Practical Identification Manual Cambridge, USA: CABI Publishing; [View Article]
    [Google Scholar]
  8. Busse H.-J., Auling G. 1988; Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . Syst Appl Microbiol 11:1–8 [View Article]
    [Google Scholar]
  9. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
    [Google Scholar]
  10. Chen Y.-G., Cui X.-L., Wang Y.-X., Tang S.-K., Zhang Y.-Q., Li W.-J., Liu J.-H., Peng Q., Xu L.-H. 2009; Psychroflexus sediminis sp. nov., a mesophilic bacterium isolated from salt lake sediment in China. Int J Syst Evol Microbiol 59:569–573 [View Article][PubMed]
    [Google Scholar]
  11. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  12. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  13. Dobson S. J., Colwell R. R., McMeekin T. A., Franzmann P. D. 1993; Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake. Int J Syst Bacteriol 43:77–83 [View Article][PubMed]
    [Google Scholar]
  14. Donachie S. P., Bowman J. P., Alam M. 2004; Psychroflexus tropicus sp. nov., an obligately halophilic Cytophaga-Flavobacterium-Bacteroides group bacterium from an Hawaiian hypersaline lake. Int J Syst Evol Microbiol 54:935–940 [View Article][PubMed]
    [Google Scholar]
  15. Euzéby J. P. 2012 List of Prokaryotic names with Standing in Nomenclature. http://www.bacterio.cict.fr/index.html
  16. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  17. Rüger H.-J., Krambeck H.-J. 1994; Evaluation of the BIOLOG substrate metabolism system for classification of marine bacteria. Syst Appl Microbiol 17:281–288 [View Article]
    [Google Scholar]
  18. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
  19. Schmidt V. S., Mayr R., Wenning M., Glöckner J., Busse H.-J., Scherer S. 2009; Bavariicoccus seileri gen. nov., sp. nov., isolated from the surface and smear water of German red smear soft cheese. Int J Syst Evol Microbiol 59:2437–2443 [View Article][PubMed]
    [Google Scholar]
  20. Scholz H. C., Hubalek Z., Sedlácek I., Vergnaud G., Tomaso H., Al Dahouk S., Melzer F., Kämpfer P., Neubauer H. other authors 2008; Brucella microti sp. nov., isolated from the common vole Microtus arvalis . Int J Syst Evol Microbiol 58:375–382 [View Article][PubMed]
    [Google Scholar]
  21. Stolz A., Busse H.-J., Kämpfer P. 2007; Pseudomonas knackmussii sp. nov.. Int J Syst Evol Microbiol 57:572–576 [View Article][PubMed]
    [Google Scholar]
  22. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  23. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  24. Tindall B. J. 1990a; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
    [Google Scholar]
  25. Tindall B. J. 1990b; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
    [Google Scholar]
  26. Tindall B. J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P. 2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266 [View Article][PubMed]
    [Google Scholar]
  27. Verbarg S., Frühling A., Cousin S., Brambilla E., Gronow S., Lünsdorf H., Stackebrandt E. 2008; Biostraticola tofi gen. nov., spec. nov., a novel member of the family Enterobacteriaceae . Curr Microbiol 56:603–608 [View Article][PubMed]
    [Google Scholar]
  28. Yamamoto S., Yoshida M., Nakao H., Koyama M., Hashimoto Y., Shinoda S. 1986; Variations in cellular polyamine compositions and contents of Vibrio species during growth in media with various NaCl concentrations. Chem Pharm Bull (Tokyo) 34:3038–3042 [View Article][PubMed]
    [Google Scholar]
  29. Yoon J.-H., Kang S.-J., Jung Y.-T., Oh T.-K. 2009; Psychroflexus salinarum sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 59:2404–2407 [View Article][PubMed]
    [Google Scholar]
  30. Zhang Z., Schwartz S., Wagner L., Miller W. 2000; A greedy algorithm for aligning DNA sequences. J Comput Biol 7:203–214 [View Article][PubMed]
    [Google Scholar]
  31. Zhang H., Hosoi-Tanabe S., Nagata S., Ban S., Imura S. 2010; Psychroflexus lacisalsi sp. nov., a moderate halophilic bacterium isolated from a hypersaline lake (Hunazoko-Ike) in Antarctica. J Microbiol 48:160–164 [View Article][PubMed]
    [Google Scholar]
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