1887

Abstract

A novel halophilic, Gram-reaction-negative, strictly aerobic, non-motile, rod-shaped and oxidase- and catalase-positive bacterial strain, designated WCC 4520, was isolated from a semi-hard, Raclette-type cheese. The colonies were yellow–orange; flexirubin-type pigments were not found. The strain hydrolysed gelatin, hippurate, tyrosine and Tweens 20 and 80. Optimal growth was observed with 6–8 % (w/v) NaCl, at pH 7–8 and at 27–30 °C. The genomic DNA G+C content was 33.6 mol%. In phylogenetic analysis based on 16S rRNA gene sequences, strain WCC 4520 appeared to be a member of the family and the closest phylogenetic neighbours were identified as DSM 5423 (94.0 % 16S rRNA gene sequence similarity) and CCUG 56752 (94.0 %). The predominant cellular fatty acids were iso-C, anteiso-C, iso-C G and iso-C 3-OH. The only detected quinone was MK-6 and the major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and an unidentified polar lipid. Minor polar lipids and traces of polyamines were also detected. On the basis of the data presented, strain WCC 4520 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WCC 4520 ( = LMG 25857 = CCUG 59705).

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2012-08-01
2020-01-26
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