A Gram-negative, non-motile, ovoid to rod-shaped bacterium, designated HDW-32T, was isolated from seawater of the Yellow Sea, Korea. Strain HDW-32T grew optimally at pH 7–8, at 30 °C and with 2–3 % (w/v) NaCl. Strain HDW-32T exhibited 95.1 % 16S rRNA gene sequence similarity with Nitratireductor basaltis J3T, 94.8 % sequence similarity with Ahrensia kielensis IAM 12618T and <94.5 % with other members of the family Phyllobacteriaceae. In the neighbour-joining, maximum-likelihood and maximum-parsimony trees based on 16S rRNA gene sequences, strain HDW-32T clustered with A. kielensis IAM 12618T. Strain HDW-32T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. Differences in polar lipids, DNA G+C content and other phenotypic properties distinguished strain HDW-32T from A. kielensis JCM 20689T. Strain HDW-32T could also be distinguished from representatives of the genera Nitratireductor and Hoeflea by differences in fatty acids and polar lipids. On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain HDW-32T represents a novel species belonging to a novel genus of the family Phyllobacteriaceae of the class Alphaproteobacteria, for which the name Pseudahrensia aquimaris gen. nov., sp. nov. is proposed. The type strain of the type species is HDW-32T ( = KCTC 23345T = CCUG 60023T).
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Funding
This study was supported by the:
Program for Collection, Management and Utilization of Biological Resources
(Award M10867010003)
21C Frontier Program of Microbial Genomics and Applications
(Award 11-2008-00-002-00)
BK21 Program from the Ministry of Education, Science and Technology (MEST)
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