gen. nov., sp. nov., isolated from seawater Free

Abstract

A Gram-negative, non-motile, ovoid to rod-shaped bacterium, designated HDW-32, was isolated from seawater of the Yellow Sea, Korea. Strain HDW-32 grew optimally at pH 7–8, at 30 °C and with 2–3 % (w/v) NaCl. Strain HDW-32 exhibited 95.1 % 16S rRNA gene sequence similarity with J3, 94.8 % sequence similarity with IAM 12618 and <94.5 % with other members of the family . In the neighbour-joining, maximum-likelihood and maximum-parsimony trees based on 16S rRNA gene sequences, strain HDW-32 clustered with IAM 12618. Strain HDW-32 contained Q-10 as the predominant ubiquinone and Cω7 as the major fatty acid. Differences in polar lipids, DNA G+C content and other phenotypic properties distinguished strain HDW-32 from JCM 20689. Strain HDW-32 could also be distinguished from representatives of the genera and by differences in fatty acids and polar lipids. On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain HDW-32 represents a novel species belonging to a novel genus of the family of the class for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is HDW-32 ( = KCTC 23345  = CCUG 60023).

Funding
This study was supported by the:
  • Program for Collection, Management and Utilization of Biological Resources (Award M10867010003)
  • 21C Frontier Program of Microbial Genomics and Applications (Award 11-2008-00-002-00)
  • BK21 Program from the Ministry of Education, Science and Technology (MEST)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.034793-0
2012-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/9/2056.html?itemId=/content/journal/ijsem/10.1099/ijs.0.034793-0&mimeType=html&fmt=ahah

References

  1. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [View Article][PubMed]
    [Google Scholar]
  2. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  3. Kang H. S., Yang H. L., Lee S. D. 2009; Nitratireductor kimnyeongensis sp. nov., isolated from seaweed. Int J Syst Evol Microbiol 59:1036–1039 [View Article][PubMed]
    [Google Scholar]
  4. Kim K.-H., Roh S. W., Chang H.-W., Nam Y.-D., Yoon J.-H., Jeon C. O., Oh H.-M., Bae J.-W. 2009; Nitratireductor basaltis sp. nov., isolated from black beach sand. Int J Syst Evol Microbiol 59:135–138 [View Article][PubMed]
    [Google Scholar]
  5. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  6. Labbé N., Parent S., Villemur R. 2004; Nitratireductor aquibiodomus gen. nov., sp. nov., a novel α-proteobacterium from the marine denitrification system of the Montreal Biodome (Canada). Int J Syst Evol Microbiol 54:269–273 [View Article][PubMed]
    [Google Scholar]
  7. Lai Q., Yu Z., Yuan J., Sun F., Shao Z. 2011a; Nitratireductor indicus sp. nov., isolated from deep-sea water. Int J Syst Evol Microbiol 61:295–298 [View Article][PubMed]
    [Google Scholar]
  8. Lai Q., Yu Z., Wang J., Zhong H., Sun F., Wang L., Wang B., Shao Z. 2011b; Nitratireductor pacificus sp. nov., isolated from a pyrene-degrading consortium. Int J Syst Evol Microbiol 61:1386–1391 [View Article][PubMed]
    [Google Scholar]
  9. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
    [Google Scholar]
  10. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184[PubMed]
    [Google Scholar]
  11. Mergaert J., Swings J. 2005; Family IV. Phyllobacteriaceae fam. nov.. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 2 p. 393 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  12. Mergaert J., Swings J. 2006; Phyllobacteriaceae fam. nov. In List of New Names and New Combinations Previously Effectively, but not Validly, Published, Validation List no. 107. Int J Syst Evol Microbiol 56:1–6 [View Article][PubMed]
    [Google Scholar]
  13. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  14. Peix A., Rivas R., Trujillo M. E., Vancanneyt M., Velázquez E., Willems A. 2005; Reclassification of Agrobacterium ferrugineum LMG 128 as Hoeflea marina gen. nov., sp. nov.. Int J Syst Evol Microbiol 55:1163–1166 [View Article][PubMed]
    [Google Scholar]
  15. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
  16. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  17. Uchino Y., Hirata A., Yokota A., Sugiyama J. 1998; Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev.. J Gen Appl Microbiol 44:201–210 [View Article][PubMed]
    [Google Scholar]
  18. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [View Article]
    [Google Scholar]
  19. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [View Article][PubMed]
    [Google Scholar]
  20. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.034793-0
Loading
/content/journal/ijsem/10.1099/ijs.0.034793-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed