1887

Abstract

Two ochratoxin A (OTA)-producing isolates, recently collected from submerged riparian decomposing leaves in Italy, were found to have a similar morphology to (subgenus , section ). However, marked differences emerged between these two novel isolates and . as the former displayed different colony features and had larger vesicles, metulae, phialides and conidia, as well as a distinct sclerotial form and size. In order to determine the taxonomic status and to infer the evolutionary relationships of these two morphologically identical isolates, a molecular phylogenetic analysis was performed on all the officially recognized lineages in the section . The DNA sequences and the deduced amino acid residues from the nuclear loci were analysed. Both rRNA and protein coding genes were assessed, which are widely used to differentiate taxa belonging to genus at various evolutionary levels. The 5.8S rDNA gene and internal transcribed spacers (ITS), the D1/D2 domains of the 28S rDNA gene, a region of the tubulin beta chain gene () and part of the calmodulin gene () were amplified by PCR and then sequenced. The analysis of the rRNA regions and of the and sequence data indicated that the two isogenic isolates belonged to a genetically distinct OTA-producing species of the genus . The isolates are proposed as representing a novel species, sp. nov., with the type strain ATCC MYA-4773( = CBS 129190 = 417). Phylogenetically, sp. nov. appeared to be very closely related to , from which it could be distinguished by means of a morphological trait analysis.

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2012-04-01
2019-10-16
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