1887

Abstract

A Gram-staining-negative, non-motile, cream-coloured and rod-shaped bacterium, designated strain LNB2, was isolated from a hexachlorocyclohexane-contaminated dump site in the village of Ummari, in northern India. The taxonomic position of the novel strain was investigated by using a polyphasic approach. In a phylogenetic analysis based on 16S rRNA gene sequences, strain LNB2 appeared to be most closely related to A175 (98.0 % sequence similarity) and UM2 (97.3 %). In DNA–DNA hybridizations, the levels of DNA–DNA relatedness between the novel strain and A175 and UM2 were found to be low (8.6 % and 5.6 %, respectively). The genomic DNA G+C content of strain LNB2 was 61.0 mol%. The novel strain’s predominant fatty acids were summed feature 8 (Cω7 and/or Cω6), C, summed feature 3 (Cω7 and/or Cω6), C 2-OH, Cω6 and 11-methyl Cω7. The major ubiquinone was Q-10, the predominant polyamine was homospermidine, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, sphingoglycolipid, phosphatidylethanolamine and phosphatidyldimethylethanolamine. Based on the phylogenetic, biochemical and chemotaxonomic evidence and the results of the DNA–DNA hybridizations, strain LNB2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LNB2 ( = MTCC 10873 = CCM 7880 = DSM 25432).

Funding
This study was supported by the:
  • Government of India
  • the National Bureau of Agriculturally Important Micro-organisms (NBAIM)
  • National Department of Science and Technology’s PURSE Initiative
  • Indian Council for Scientific and Industrial Research
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2012-12-01
2024-03-29
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