1887

Abstract

A novel mesophilic, strictly anaerobic bacterium, strain BM, was isolated from food industry wastewater. The cells were motile, non-spore-forming rods and stained Gram-negative. Growth of strain BM was observed at 16–44 °C (optimum 37 °C) and pH 6.0–9.0 (optimum pH 7.5). The NaCl concentration range for growth was 0–8 % (optimum 1.5 %, w/v). Strain BM was chemo-organotrophic, using a few sugars and amino acids as sole carbon and energy sources. The fermentation products from peptone-yeast extract broth were propionate, formate, acetate, ethanol and isovalerate. Indole, NH and HS were produced from peptone. No respiratory quinones could be detected. The major fatty acids were iso-C (39.3 %), iso-C dimethyl acetal (10.1 %), anteiso-C (7.6 %), C (6.1 %) and C (5.6 %). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol and a number of unidentified aminoglycolipids, glycolipids and phospholipids. The DNA G+C content was 28.2 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain BM was related to various genera of the family , and its closest relatives were SOL3f37 (94.3 % 16S rRNA gene sequence similarity), GW (92.1 %) and 37HS60 (92.0 %). In recognition of its distinct phenotypic and genotypic characteristics, isolate BM is proposed to represent a novel species of a new genus, gen. nov., sp. nov. The type strain of is BM ( = JCM 16868  = DSM 24659).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30970002)
  • National Science and Technology Major Projects – Water Pollution Control and Treatment Project (Award 2008ZX07101-006-3)
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2012-12-01
2021-10-21
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