RT Journal Article SR Electronic(1) A1 Pugin, Benoît A1 Blamey, Jenny M. A1 Baxter, Bonnie K. A1 Wiegel, JuergenYR 2012 T1 Amphibacillus cookii sp. nov., a facultatively aerobic, spore-forming, moderately halophilic, alkalithermotolerant bacterium JF International Journal of Systematic and Evolutionary Microbiology, VO 62 IS Pt_9 SP 2090 OP 2096 DO https://doi.org/10.1099/ijs.0.034629-0 PB Microbiology Society, SN 1466-5034, AB Novel strains of facultatively aerobic, moderately alkaliphilic and facultatively halophilic bacteria were isolated from a sediment sample taken from the Southern Arm of Great Salt Lake, Utah. Cells of strain JW/BP-GSL-QDT (and related strains JW/BP-GSL-RA and JW/BP-GSL-WB) were rod-shaped, spore-forming, motile bacteria with variable Gram-staining. Strain JW/BP-GSL-QDT grew under aerobic conditions between 14.5 and 47 °C (optimum 39 °C), in the pH37 °C range 6.5–10.3 (optimum pH37 °C 8.0), and between 0.1 and 4.5 M Na+ (optimum 0.9 M Na+). No growth was observed in the absence of supplemented Na+. Strain JW/BP-GSL-QDT utilized l-arabinose, d-fructose, d-galactose, d-glucose, inulin, lactose, maltose, mannitol, d-mannose, pyruvate, d-ribose, d-sorbitol, starch, trehalose, xylitol and d-xylose under both aerobic and anaerobic conditions, and used ethanol and methanol only under aerobic conditions. Strains JW/BP-GSL-WB and JW/BP-GSL-RA had the same profiles except that methanol was not used aerobically. During growth on glucose, the major organic compounds formed under aerobic conditions were acetate and lactate, and under anaerobic conditions, the fermentation products were formate, acetate, lactate and ethanol. Oxidase and catalase activities were not detected and cytochrome was absent. No respiratory quinones were detected. The main cellular fatty acids were iso-C15 : 0 (39.1 %) and anteiso-C15 : 0 (36.3 %). Predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unknown phospholipid. Additionally, a small amount of an unknown glycolipid was detected. The DNA G+C content of strain JW/BP-GSL-QDT was 35.4 mol% (determined by HPLC). For strain JW/BP-GSL-QDT the highest degree of 16S rRNA gene sequence similarity was found with Amphibacillus jilinensis (98.6 %), Amphibacillus sediminis (96.7 %) and Amphibacillus tropicus (95.6 %). The level of DNA–DNA relatedness between strain JW/BP-GSL-QDT and A. jilinensis Y1T was 58 %. On the basis of physiological, chemotaxonomic and phylogenetic data, strain JW/BP-GSL-QDT represents a novel species of the genus Amphibacillus , for which the name Amphibacillus cookii sp. nov. is proposed. The type strain is JW/BP-GSL-QDT ( = ATCC BAA-2118T = DSM 23721T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.034629-0