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Volume 62, Issue Pt_9

sp. nov., a facultatively aerobic, spore-forming, moderately halophilic, alkalithermotolerant bacterium Free

Abstract

Novel strains of facultatively aerobic, moderately alkaliphilic and facultatively halophilic bacteria were isolated from a sediment sample taken from the Southern Arm of Great Salt Lake, Utah. Cells of strain JW/BP-GSL-QD (and related strains JW/BP-GSL-RA and JW/BP-GSL-WB) were rod-shaped, spore-forming, motile bacteria with variable Gram-staining. Strain JW/BP-GSL-QD grew under aerobic conditions between 14.5 and 47 °C (optimum 39 °C), in the pH range 6.5–10.3 (optimum pH 8.0), and between 0.1 and 4.5 M Na (optimum 0.9 M Na). No growth was observed in the absence of supplemented Na. Strain JW/BP-GSL-QD utilized -arabinose, -fructose, -galactose, -glucose, inulin, lactose, maltose, mannitol, -mannose, pyruvate, -ribose, -sorbitol, starch, trehalose, xylitol and -xylose under both aerobic and anaerobic conditions, and used ethanol and methanol only under aerobic conditions. Strains JW/BP-GSL-WB and JW/BP-GSL-RA had the same profiles except that methanol was not used aerobically. During growth on glucose, the major organic compounds formed under aerobic conditions were acetate and lactate, and under anaerobic conditions, the fermentation products were formate, acetate, lactate and ethanol. Oxidase and catalase activities were not detected and cytochrome was absent. No respiratory quinones were detected. The main cellular fatty acids were iso-C (39.1 %) and anteiso-C (36.3 %). Predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unknown phospholipid. Additionally, a small amount of an unknown glycolipid was detected. The DNA G+C content of strain JW/BP-GSL-QD was 35.4 mol% (determined by HPLC). For strain JW/BP-GSL-QD the highest degree of 16S rRNA gene sequence similarity was found with (98.6 %), (96.7 %) and (95.6 %). The level of DNA–DNA relatedness between strain JW/BP-GSL-QD and Y1 was 58 %. On the basis of physiological, chemotaxonomic and phylogenetic data, strain JW/BP-GSL-QD represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JW/BP-GSL-QD ( = ATCC BAA-2118 = DSM 23721).

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Funding
This study was supported by the:
  • US Airforce Office of Scientific Research (Award AFOSR 033835-01)
  • National Science Foundation (Award MCB 0604224)
© 2012 IUMS
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2012-09-01
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Amphibacillus cookii sp. nov., a facultatively aerobic, spore-forming, moderately halophilic, alkalithermotolerant bacterium
Int J Syst Evol Microbiol 62, 2090 (2012); https://doi.org/10.1099/ijs.0.034629-0
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