Strain BELH1T, a novel mesophilic, anaerobic, halotolerant, rod-shaped bacterium, was isolated from a Tunisian wastewater digester. The cells of the strain are motile, measure 0.5×2–5 µm, and occur singly or in pairs. The strain reduced thiosulfate and elemental sulfur (but not sulfate or sulfite) into sulfide. It grew at 15–40 °C (optimum 30 °C), pH 5.8–8.4 (optimum 7) and with 0–10 % (w/v) NaCl (optimum 3.0 %). The genomic DNA G+C content of strain BELH1T was 38.2 mol% and the strain’s predominant cellular fatty acids were C14 : 0, asummed feature that contained iso-C17 : 1 and/or anteiso-C17 : 1 B, and C16 : 0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain was most closely related to Fusibacter paucivorans (94.8 % sequence similarity). Based on phenotypic, phylogenetic and taxonomic characteristics, strain BELH1T represents a novel species of the genus Fusibacter, for which the name Fusibacter tunisiensis sp. nov. is proposed. The type strain is BELH1T ( = DSM 24436T = JCM 17481T).
CashionP.,
Holder-FranklinM. A.,
McCullyJ.,
FranklinM.1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
Cord-RuwischR.1985; A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. J Microbiol Methods 4:33–36 [View Article]
HallT. A.1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
KhelifiN.,
Ben RomdhaneE.,
HediA.,
PostecA.,
FardeauM. L.,
HamdiM.,
TholozanJ. L.,
OllivierB.,
Hirschler-RéaA.2010; Characterization of Microaerobacter geothermalis gen. nov., sp. nov., a novel microaerophilic, nitrate- and nitrite-reducing thermophilic bacterium isolated from a terrestrial hot spring in Tunisia. Extremophiles 14:297–304 [View Article][PubMed]
KuykendallL. D.,
RoyM. A.,
O'NeilJ. J.,
DevineT. E.1988; Fatty acids, antibiotic resistance, and desoxyribonucleic acid homology groups of Bradyrhizobium japonicum
. Int J Syst Bacteriol 38:358–361 [View Article]
MesbahM.,
PremachandranU.,
WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
RavotG.,
MagotM.,
FardeauM.-L.,
PatelB. K. C.,
ThomasP.,
GarciaJ.-L.,
OllivierB.1999; Fusibacter paucivorans gen. nov., sp. nov., an anaerobic, thiosulfate-reducing bacterium from an oil-producing well. Int J Syst Bacteriol 49:1141–1147 [View Article][PubMed]
Van de PeerY.,
De WachterR.1994; treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570[PubMed]
WiddelF.,
PfennigN.1982; Studies on dissimilatory sulphate-reducing bacteria that decompose fatty acids II. Incomplete oxidation of propionate by Desulfobulbus propionicus gen. nov., sp. nov.. Arch Microbiol 131:360–365 [View Article]