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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 62, Issue Pt_8

sp. nov., an effective symbiotic bacterium isolated from soybean ( L.) nodules Free

Abstract

In a survey of the biodiversity and biogeography of rhizobia associated with soybean ( L.) in different sites of the Northern (Huang-Huai-Hai) Plain of China, ten strains were defined as representing a novel genomic species in the genus of . They were distinguished from defined species in restriction fragment length polymorphism (RFLP) analysis of the 16S rRNA gene and the 16S–23S rRNA gene intergenic spacer (IGS). In BOX-PCR, these strains presented two patterns that shared 94 % similarity, demonstrating that they were a homogenous group with limited diversity. In phylogenetic analyses of the 16S rRNA gene, IGS and housekeeping gene sequences, four representative strains formed a distant lineage within the genus , which was consistent with the results of DNA–DNA hybridization. The strains of this novel group formed effective nodules with , and in cross-nodulation tests and harboured symbiotic genes ( and ) identical to those of reference strains of , and ‘’ originating from soybean, implying that the novel group may have obtained these symbiotic genes by lateral gene transfer. In analyses of cellular fatty acids and phenotypic features, some differences were found between the novel group and related species, demonstrating that the novel group is distinct phenotypically from other species. Based upon the data obtained, these strains are proposed to represent a novel species, sp. nov., with CCBAU 23303 ( = LMG 26136  = CGMCC 1.10948  = HAMBI 3180) as the type strain. The DNA G+C content of strain CCBAU 23303 is 61.5 mol% ( ).

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Funding
This study was supported by the:
  • Foundation of the State Key Basic Research and Development Plan of China (Award 2010CB126500)
  • Funds of Commercialization of Agricultural and Scientific Findings, MOST (Award 2008GB23600460)
  • National Natural Science Foundation of China (Award 30870004 and 30970004)
  • SKLAB (Award 20110423, SIP20100067, 2010SKLAB01-1 and 2009SKLAB05-1)
  • IPN (Award PICS08-3)
  • ICyT DF of Mexico
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Bradyrhizobium huanghuaihaiense sp. nov., an effective symbiotic bacterium isolated from soybean (Glycine max L.) nodules
Int J Syst Evol Microbiol 62, 1951 (2012); https://doi.org/10.1099/ijs.0.034546-0
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