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Volume 62, Issue Pt_7

sp. nov., isolated from the profundal sediment of a freshwater meromictic lake Free

Abstract

An autotrophic, hydrogenotrophic methanogen, designated strain 17A1, was isolated from the profundal sediment of the meromictic Lake Pavin, France. The cells of the novel strain, which were non-motile, Gram-staining-negative rods that measured 2–15 µm in length and 0.2–0.4 µm in width, grew as filaments. Strain 17A1 grew in a mineral medium and its growth was stimulated by the addition of yeast extract, vitamins, acetate or rumen fluid. Penicillin, vancomycin and kanamycin reduced growth but did not completely inhibit it. Growth occurred at 14–41 °C (optimum 30 °C), at pH 5.0–8.5 (optimum pH 6.5) and with 0–0.4 M NaCl (optimum 0.1 M). The novel strain utilized H/CO and methanol/H as substrates but not formate, acetate, methylamine/H, isobutanol or 2-propanol. Its genomic DNA G+C content was 37.0 mol%. In phylogenetic analyses based on 16S rRNA gene sequences, strain 17A1 appeared to be a member of the genus , with 8-2 (96.3 % sequence similarity) identified as the most closely related established species. Based on phenotypic and phylogenetic data, strain 17A1 represents a novel species of methanogen within the genus , for which the name sp. nov. is proposed. The type strain is 17A1 ( = DSM 24406 = JCM 17760).

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Funding
This study was supported by the:
  • Region Auvergne
  • Centre National de la Recherche Scientifique
© 2012 IUMS
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2012-07-01
2024-04-16
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410[PubMed] [CrossRef]
     [Google Scholar]
  2. Assayag N., Jézéquel D., Ader M., Viollier E., Michard G., Prévot F., Agrinier P. 2008; Hydrological budget, carbon sources and biogeochemical processes in Lac Pavin (France): constraints from δ18O of water and δ13C of dissolved inorganic carbon. Appl Geochem 23:2800–2816 [View Article]
     [Google Scholar]
  3. Bastviken D., Cole J., Pace M., Tranvik L. 2004; Methane emissions from lakes: dependence of lake characteristics, two regional assessments, and a global estimate. Global Biogeochem Cycles 18:GB4009 [View Article]
     [Google Scholar]
  4. Bonin A. S., Boone D. 2006; The order Methanobacteriales . In The Prokaryotes pp. 231–243 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York: Springer; [View Article]
     [Google Scholar]
  5. Bryant M. P., Boone D. R. 1987; Isolation and characterization of Methanobacterium formicicum MF. Int J Syst Bacteriol 37:171–171 [View Article]
     [Google Scholar]
  6. Chan O. C., Wolf M., Hepperle D., Casper P. 2002; Methanogenic archaeal community in the sediment of an artificially partitioned acidic bog lake. FEMS Microbiol Ecol 42:119–129 [View Article][PubMed]
     [Google Scholar]
  7. Chavant P., Taupin V., El Alaoui H., Wawrzyniak I., Chambon C., Prensier G., Méténier G., Vivarès C. P. 2005; Proteolytic activity in Encephalitozoon cuniculi sporogonial stages: predominance of metallopeptidases including an aminopeptidase-P-like enzyme. Int J Parasitol 35:1425–1433 [View Article][PubMed]
     [Google Scholar]
  8. Conrad R. 1999; Contribution of hydrogen to methane production and control of hydrogen concentrations in methanogenic soils and sediments. FEMS Microbiol Ecol 28:193–202 [View Article]
     [Google Scholar]
  9. DeLong E. F. 1992; Archaea in coastal marine environments. Proc Natl Acad Sci U S A 89:5685–5689 [View Article][PubMed]
     [Google Scholar]
  10. Hungate R. E. 1969; A roll tube method for cultivation of strict anaerobes. Methods Microbiol 3B:117–132 [View Article]
     [Google Scholar]
  11. Kitamura K., Fujita T., Akada S., Tonouchi A. 2011; Methanobacterium kanagiense sp. nov., a hydrogenotrophic methanogen, isolated from Japanese rice field soil. Int J Syst Evol Microbiol 61:1246–1252 [CrossRef]
     [Google Scholar]
  12. Krivushin K. V., Shcherbakova V. A., Petrovskaya L. E., Rivkina E. M. 2010; Methanobacterium veterum sp. nov., from ancient Siberian permafrost. Int J Syst Evol Microbiol 60:455–459 [View Article][PubMed]
     [Google Scholar]
  13. Lehours A. C., Evans P., Bardot C., Joblin K., Gérard F. 2007; Phylogenetic diversity of archaea and bacteria in the anoxic zone of a meromictic lake (Lake Pavin, France). Appl Environ Microbiol 73:2016–2019 [View Article][PubMed]
     [Google Scholar]
  14. Lehours A. C., Rabiet M., Morel-Desrosiers N., Morel J. P., Jouve L., Arbeille B., Mailhot G., Fonty G. 2010; Ferric iron reduction by fermentative strain BS2 isolated from an iron-rich anoxic environment (Lake Pavin, France). Geomicrobiol J 27:714–722 [View Article]
     [Google Scholar]
  15. Lomans B. P., Maas R., Luderer R., Op den Camp H. J. M., Pol A., van der Drift C., Vogels G. D. 1999; Isolation and characterization of Methanomethylovorans hollandica gen. nov., sp. nov., isolated from freshwater sediment, a methylotrophic methanogen able to grow on dimethyl sulfide and methanethiol. Appl Environ Microbiol 65:3641–3650[PubMed]
     [Google Scholar]
  16. Ma K., Liu X. L., Dong X. Z. 2005; Methanobacterium beijingense sp. nov., a novel methanogen isolated from anaerobic digesters. Int J Syst Evol Microbiol 55:325–329 [View Article][PubMed]
     [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
     [Google Scholar]
  18. Michard G., Viollier E., Jezequel D., Sarazin G. 1994; Geochemical study of a crater lake: Pavin Lake, France – identification, location and quantification of the chemical reactions in the lake. Chem Geol 115:103–115 [View Article]
     [Google Scholar]
  19. Mink R. W., Dugan P. R. 1977; Tentative identification of methanogenic bacteria by fluorescence microscopy. Appl Environ Microbiol 33:713–717[PubMed]
     [Google Scholar]
  20. Mori K., Harayama S. 2011; Methanobacterium petrolearium sp. nov. and Methanobacterium ferruginis sp. nov., mesophilic methanogens isolated from salty environments. Int J Syst Evol Microbiol 61:138–143 [View Article][PubMed]
     [Google Scholar]
  21. Nüsslein B., Chin K.-J., Eckert W., Conrad R. 2001; Evidence for anaerobic syntrophic acetate oxidation during methane production in the profundal sediment of subtropical Lake Kinneret (Israel). Environ Microbiol 3:460–470 [View Article][PubMed]
     [Google Scholar]
  22. Patel G. B., Sprott G. D., Fein J. E. 1990; Isolation and characterization of Methanobacterium espanolae sp. nov., a mesophilic, moderately acidiphilic methanogen. Int J Syst Bacteriol 40:12–18 [View Article]
     [Google Scholar]
  23. Shcherbakova V., Rivkina E., Pecheritsyna S., Laurinavichius K., Suzina N., Gilichinsky D. 2011; Methanobacterium arcticum sp. nov., a methanogenic archaeon from Holocene Arctic permafrost. Int J Syst Evol Microbiol 61:144–147 [View Article][PubMed]
     [Google Scholar]
  24. Shlimon A. G., Friedrich M. W., Niemann H., Ramsing N. B., Finster K. 2004; Methanobacterium aarhusense sp. nov., a novel methanogen isolated from a marine sediment (Aarhus Bay, Denmark). Int J Syst Evol Microbiol 54:759–763 [View Article][PubMed]
     [Google Scholar]
  25. Simankova M. V., Parshina S. N., Tourova T. P., Kolganova T. V., Zehnder A. J. B., Nozhevnikova A. N. 2001; Methanosarcina lacustris sp. nov., a new psychrotolerant methanogenic archaeon from anoxic lake sediments. Syst Appl Microbiol 24:362–367 [View Article][PubMed]
     [Google Scholar]
  26. Skillman L. C., Evans P. N., Naylor G. E., Morvan B., Jarvis G. N., Joblin K. N. 2004; 16S ribosomal DNA-directed PCR primers for ruminal methanogens and identification of methanogens colonising young lambs. Anaerobe 10:277–285 [View Article][PubMed]
     [Google Scholar]
  27. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
     [Google Scholar]
  28. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
     [Google Scholar]
  29. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
     [Google Scholar]
  30. Zellner G., Bleicher K., Braun E., Kneifel H., Tindall B. J., Macario E. C., Winter J. 1988; Characterization of a new mesophilic, secondary alcohol-utilizing methanogen, Methanobacterium palustre spec. nov. from a peat bog. Arch Microbiol 151:1–9 [View Article]
     [Google Scholar]
  31. Zhu J., Liu X., Dong X. 2011; Methanobacterium movens sp. nov. and Methanobacterium flexile sp. nov., isolated from lake sediment. Int J Syst Evol Microbiol 61:2974–2978 [View Article][PubMed]
     [Google Scholar]
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Methanobacterium lacus sp. nov., isolated from the profundal sediment of a freshwater meromictic lake
Int J Syst Evol Microbiol 62, 1625 (2012); https://doi.org/10.1099/ijs.0.034538-0
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