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Abstract

An orange-pigmented, Gram-reaction-positive, non-spore-forming, halophilic, alkali-tolerant rod, designated strain halo-2, was isolated from sediment of Xiarinaoer soda lake, in China’s Inner Mongolia Autonomous Region. Strain halo-2 grew in a complex medium with 3–30 % (w/v) NaCl and at pH 5–10. The cell-wall peptidoglycan contained -diaminopimelic acid and the major respiratory isoprenoid quinone was MK-7. The predominant cellular fatty acids were anteiso-C (43.6 %), anteiso-C (14.8 %) and iso-C (6.8 %) and the polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The genomic DNA G+C content of the novel strain was 48.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain halo-2 was most closely related to DSM 8721 (93.9 % sequence similarity). However, strain halo-2 could be clearly differentiated from its closest phylogenetic relatives on the basis of several phenotypic, genotypic and chemotaxonomic characteristics. Strain halo-2 therefore represents a novel species in a new genus for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is halo-2 ( = CGMCC 1.7654 = NBRC 104935).

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 31070105)
  • , National Public Benefit Research Sector of China’s Ministry of Land and Resources , (Award 200911043)
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2012-09-01
2020-10-23
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