1887

Abstract

An aerobic, motile, moderately thermophilic, rod-shaped bacterium, strain YBY-7, was isolated from a terrestrial hot spring of a garden exhibition located in Xiamen City, Fujian Province, People’s Republic of China. Cells of strain YBY-7 were Gram-negative, irregular rods, 2–6 µm long and 0.4–0.6 µm wide, with polar flagella, and the organism formed beige colonies. The temperature and pH ranges for growth of strain YBY-7 were 28–65 °C (optimum 50–58 °C) and pH 6.5–9.5 (optimum pH 7.5–8.5). Growth occurred in the presence of 5.5 % NaCl (optimum 3.0 %). Phylogenetic analysis based on the 16S rRNA gene sequence revealed that FRR-10 was its closest neighbour (95.9 % similarity). Ubiquinone (Q-10) was the sole respiratory quinone and the DNA G+C content of strain YBY-7 was 70.6 mol%. The predominant polar lipids were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were Cω7 (29.3 %), C cyclo ω8 (25.6 %), C (23.6 %) and C (9.6 %). Based on its physiological characteristics and our phylogenetic analysis, we propose that strain YBY-7 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain YBY-7 ( = MCCC 1A06317  = CGMCC 1.10789  = LMG 26247).

Funding
This study was supported by the:
  • , Scientific Research Project of Marine Public Welfare Industry of China , (Award 201205020 and 200805032)
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2012-07-01
2020-09-29
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References

  1. Albuquerque L., Santos J., Travassos P., Nobre M. F., Rainey F. A., Wait R., Empadinhas N., Silva M. T., da Costa M. S. 2002; Albidovulum inexpectatum gen. nov., sp. nov., a nonphotosynthetic and slightly thermophilic bacterium from a marine hot spring that is very closely related to members of the photosynthetic genus Rhodovulum . Appl Environ Microbiol 68:4266–4273 [CrossRef][PubMed]
    [Google Scholar]
  2. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [CrossRef][PubMed]
    [Google Scholar]
  3. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef][PubMed]
    [Google Scholar]
  4. Collins M. D., Jones D. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48:459–470 [CrossRef]
    [Google Scholar]
  5. Collins M. D., Jones D., Goodfellow M., Minnikin D. E. 1979; Isoprenoid quinone composition as a guide to the classification of Listeria, Brochothrix, Erysipelothrix and Caryophanon . J Gen Microbiol 111:453–457[PubMed] [CrossRef]
    [Google Scholar]
  6. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef][PubMed]
    [Google Scholar]
  7. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr A 479:297–306 [CrossRef][PubMed]
    [Google Scholar]
  8. Minnikin D. E., Collins M. D., Goodfellow M. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95 [CrossRef]
    [Google Scholar]
  9. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46:1088–1092 [CrossRef][PubMed]
    [Google Scholar]
  10. Rzhetsky A., Nei M. 1993; Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10:1073–1095[PubMed]
    [Google Scholar]
  11. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  12. Sasser M. 2001; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
  13. Shieh W. Y., Chen Y. W., Chaw S. M., Chiu H. H. 2003; Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol 53:479–484 [CrossRef][PubMed]
    [Google Scholar]
  14. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef][PubMed]
    [Google Scholar]
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