1887

Abstract

A Gram-negative, rod-shaped, non-spore-forming bacterium, isolated from placental tissue of a cow, was investigated for its taxonomic position. On the basis of 16S rRNA gene sequence similarities, strain UK34/07-5 was shown to belong to the class , closely related to the type strain of (96.0 % sequence similarity). The polyamine pattern showed the major compound spermidine and moderate amounts of putrescine. The major quinone was ubiquinone Q-10. The polar lipid profile was composed of the major compounds phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylmonomethylethanolamine and moderate amounts of diphosphatidylglycerol, three unidentified aminolipids and an unidentified phospholipid. The profile of major fatty acids, consisting of C cyclo ω8 and Cω7, with C 3-OH as the hydroxylated fatty acid, was very similar to that of M 2040. The results of DNA–DNA hybridization and physiological and biochemical tests allowed both genotypic and phenotypic differentiation of the isolate from . The relatively low 16S rRNA gene sequence similarity of 96.0 % to M 2040 and marked differences in the polar lipid profiles as well as the results of physiological tests and the DNA–DNA hybridization data support the creation of a novel species, for which the name sp. nov. is proposed, with the type strain UK34/07-5 ( = CIP 110303  = CCUG 61094  = DSM 24741  = CCM 7941).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.034389-0
2012-05-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/5/1117.html?itemId=/content/journal/ijsem/10.1099/ijs.0.034389-0&mimeType=html&fmt=ahah

References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. 1996; Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47:39–52 [View Article]
    [Google Scholar]
  2. Busse H.-J., Auling G. 1988; Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . Syst Appl Microbiol 11:1–8 [View Article]
    [Google Scholar]
  3. Farrell I. D. 1974; The development of a new selective medium for the isolation of Brucella abortus from contaminated sources. Res Vet Sci 16:280–286[PubMed]
    [Google Scholar]
  4. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [View Article]
    [Google Scholar]
  6. Kämpfer P., Steiof M., Dott W. 1991; Microbiological characterisation of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [View Article]
    [Google Scholar]
  7. Kämpfer P., Dreyer U., Neef A., Dott W., Busse H.-J. 2003; Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53:93–97 [View Article][PubMed]
    [Google Scholar]
  8. Kämpfer P., Scholz H. C., Langer S., Wernery U., Wernery R., Johnson B., Joseph M., Lodders N., Busse H.-J. 2010; Camelimonas lactis gen. nov., sp. nov., isolated from the milk of camels. Int J Syst Evol Microbiol 60:2382–2386 [View Article][PubMed]
    [Google Scholar]
  9. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  10. Olsen G. J., Matsuda H., Hagström R., Overbeek R. 1994; fastDNAmL: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput Appl Biosci 10:41–48[PubMed]
    [Google Scholar]
  11. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. 2007; silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb . Nucleic Acids Res 35:7188–7196 [View Article][PubMed]
    [Google Scholar]
  12. Stolz A., Busse H.-J., Kämpfer P. 2007; Pseudomonas knackmussii sp. nov.. Int J Syst Evol Microbiol 57:572–576 [View Article][PubMed]
    [Google Scholar]
  13. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  14. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  15. Tindall B. J. 1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
    [Google Scholar]
  16. Tindall B. J. 1990b; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
    [Google Scholar]
  17. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R. 1998; Reclassification of Shewanella putrefaciens Owen’s genomic group II as Shewanella baltica sp. nov.. Int J Syst Bacteriol 48:179–186 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.034389-0
Loading
/content/journal/ijsem/10.1099/ijs.0.034389-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error