1887

Abstract

A symbiotic bacterium, strain IMI 397775, was isolated from the insect-pathogenic nematode . On the basis of 16S rRNA gene sequence similarity, this bacterial isolate was shown to belong to the genus , in agreement with the genus of its nematode host. The accurate phylogenetic position of this new isolate was defined using a multigene approach and showed that isolate IMI 397775 shares a common ancestor with FRM16 and PR06-A, the symbiotic bacteria associated with and , respectively. The nucleotide identity (less than 97 %) between isolate IMI 397775, FRM16 and PR06-A calculated for the concatenated sequences of five gene fragments encompassing 4275 nt, several phenotypic traits and the difference between the upper temperatures that limit growth of these three bacteria allowed genetic and phenotypic differentiation of isolate IMI 397775 from the two closely related species. Strain IMI 397775 therefore represents a novel species, for which the name sp. nov. is proposed, with the type strain IMI 397775 ( = DSM 24915).

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2012-08-01
2019-10-20
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