A Gram-staining-positive, moderately halophilic bacterium, designated strain BH097T, was isolated from solar saltern soil of Bigeum Island in south-west Korea. Cells were motile rods, producing spherical endospores at a terminal position in swollen sporangia. Strain BH097T was strictly aerobic, grew at pH 5.5–9.5 (optimum, pH 8.0), at 10–52 °C (optimum, 37 °C) and at salinities of 1–22 % (w/v) NaCl (optimum, 7 % NaCl). On the basis of 16S rRNA gene sequence analysis, strain BH097T was shown to belong to the genus Gracilibacillus within the phylum Firmicutes, and showed closest sequence similarity to Gracilibacillus saliphilus DSM 19802T (95.8 %), Gracilibacillus thailandensis TP2-8T (95.6 %), Gracilibacillus boraciitolerans DSM 17256T (95.5 %), ‘Gracilibacillus quinghaiensis’ DSM 17858 (95.4 %) and Gracilibacillus halophilus DSM 17856T (95.2 %). The DNA G+C content of this novel isolate was 37.9 mol%. The major cellular fatty acids of strain BH097T were anteiso-C15 : 0, iso-C15 : 0 and C16 : 0, and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol two unknown phospholipids and a glycolipid. The isoprenoid quinone was MK-7, and the peptidoglycan type was A1γ, with meso-diaminopimelic acid as the diagnostic diamino acid. On the basis of polyphasic evidence from this study, strain BH097T represents a novel species of the genus Gracilibacillus for which the name Gracilibacillusbigeumensis sp. nov. is proposed. The type strain is BH097T ( = KCTC 13130T = DSM 19028T).
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Funding
This study was supported by the:
Korean Ministry of Education, Science & Technology (MEST)
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