1887

Abstract

A Gram-staining-negative, aerobic, rod-shaped bacterium (CF5-1) was isolated from Hongshan Iron Mine, Daye City, Hubei province, China. The major cellular fatty acids (>10 %) were iso-C, iso-C, Cω7 alcohol and iso-Cω9. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major respiratory quinone was Q-8. The genomic DNA G+C content was 70.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CF5-1 was most closely related to (95.3 % gene sequence similarity), (94.7 %), (94.6 %) and (94.5 %). A taxonomic study using a polyphasic approach showed that strain CF5-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CF5-1 ( = CGMCC 1.10787 = KCTC 23460 = CCTCC AB 2010449).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.034132-0
2012-08-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/8/1744.html?itemId=/content/journal/ijsem/10.1099/ijs.0.034132-0&mimeType=html&fmt=ahah

References

  1. Aslam Z. , Park J. H. , Kim S. W. , Jeon C. O. , Chung Y. R. . ( 2009; ). Arenimonas oryziterrae sp. nov., isolated from a field of rice (Oryza sativa L.) managed under a no-tillage regime, and reclassification of Aspromonas composti as Arenimonas composti comb. nov.. Int J Syst Evol Microbiol 59:, 2967–2972. [CrossRef] [PubMed]
    [Google Scholar]
  2. Breznak J. A. , Costilow R. N. . ( 1994; ). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137–154. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  3. Busse H.-J. , Kämpfer P. , Moore E. R. B. , Nuutinen J. , Tsitko I. V. , Denner E. B. , Vauterin L. , Valens M. , Rosselló-Mora R. , Salkinoja-Salonen M. S. . ( 2002; ). Thermomonas haemolytica gen. nov., sp. nov., a γ-proteobacterium from kaolin slurry. . Int J Syst Evol Microbiol 52:, 473–483.[PubMed]
    [Google Scholar]
  4. Chun J. , Lee J. H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cowan S. T. , Steel K. J. . ( 1965; ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  6. Dussault H. P. . ( 1955; ). An improved technique for staining red halophilic bacteria. . J Bacteriol 70:, 484–485.[PubMed]
    [Google Scholar]
  7. Fan H. , Su C. , Wang Y. , Yao J. , Zhao K. , Wang Y. , Wang G. . ( 2008; ). Sedimentary arsenite-oxidizing and arsenate-reducing bacteria associated with high arsenic groundwater from Shanyin, Northwestern China. . J Appl Microbiol 105:, 529–539. [CrossRef] [PubMed]
    [Google Scholar]
  8. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  9. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  10. Guindon S. , Lethiec F. , Duroux P. , Gascuel O. . ( 2005; ). phyml online–a web server for fast maximum likelihood-based phylogenetic inference. . Nucleic Acids Res 33: (Web Server issue), W557–W559. [CrossRef] [PubMed]
    [Google Scholar]
  11. Hugh R. , Leifson E. . ( 1953; ). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. . J Bacteriol 66:, 24–26.[PubMed]
    [Google Scholar]
  12. Jin L. , Kim K. K. , Im W. T. , Yang H. C. , Lee S. T. . ( 2007; ). Aspromonas composti gen. nov., sp. nov., a novel member of the family Xanthomonadaceae . . Int J Syst Evol Microbiol 57:, 1876–1880. [CrossRef] [PubMed]
    [Google Scholar]
  13. Johansen J. E. , Binnerup S. J. , Kroer N. , Mølbak L. . ( 2005; ). Luteibacter rhizovicinus gen. nov., sp. nov., a yellow-pigmented gammaproteobacterium isolated from the rhizosphere of barley (Hordeum vulgare L.). . Int J Syst Evol Microbiol 55:, 2285–2291. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kämpfer P. , Martin E. , Lodders N. , Langer S. , Schumann P. , Jäckel U. , Busse H.-J. . ( 2010; ). Pseudofulvimonas gallinarii gen. nov., sp. nov., a new member of the family Xanthomonadaceae . . Int J Syst Evol Microbiol 60:, 1427–1431. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kroppenstedt R. M. . ( 1985; ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics (SAB Technical Series no. 20), pp. 173–199. Edited by Goodfellow M. , Minnikin D. E. . . London:: Academic Press;.
    [Google Scholar]
  16. Kwon S. W. , Kim B. Y. , Weon H. Y. , Baek Y. K. , Go S. J. . ( 2007; ). Arenimonas donghaensis gen. nov., sp. nov., isolated from seashore sand. . Int J Syst Evol Microbiol 57:, 954–958. [CrossRef] [PubMed]
    [Google Scholar]
  17. Lányí B. . ( 1987; ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  18. Lapage S. P. , Sneath P. H. A. , Lessel E. F. , Skerman V. B. D. , Seeliger H. P. R. , Clark W. A. . (editors) ( 1992; ). International Code of Nomenclature of Bacteria (1990 Revision). Bacteriological Code. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  19. Lee E. M. , Jeon C. O. , Choi I. , Chang K.-S. , Kim C.-J. . ( 2005; ). Silanimonas lenta gen. nov., sp. nov., a slightly thermophilic and alkaliphilic gammaproteobacterium isolated from a hot spring. . Int J Syst Evol Microbiol 55:, 385–389. [CrossRef] [PubMed]
    [Google Scholar]
  20. Lee M. , Woo S.-G. , Chae M. , Shin M.-C. , Jung H.-M. , Ten L. N. . ( 2011; ). Stenotrophomonas daejeonensis sp. nov., isolated from sewage. . Int J Syst Evol Microbiol 61:, 598–604. [CrossRef] [PubMed]
    [Google Scholar]
  21. Lim C. K. , Cooksey D. A. . ( 1993; ). Characterization of chromosomal homologs of the plasmid-borne copper resistance operon of Pseudomonas syringae . . J Bacteriol 175:, 4492–4498.[PubMed]
    [Google Scholar]
  22. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  23. Ryu E. . ( 1938; ). On the Gram-differentiation of bacteria by the simplest method. . J Jpn Soc Vet Sci 17:, 58–63.[CrossRef]
    [Google Scholar]
  24. Saddler G. S. , Bradbury J. F. . ( 2005; ). Family I. Xanthomonadaceae fam. nov.. In Bergey’s Manual of Systematic Bacteriology, (The Proteobacteria) part B (The Gammaproteobacteria), , 2nd edn., vol. 2, p. 63. Edited by Brenner D. J. , Krieg N. R. , Staley J. T. , Garrity G. M. . . New York:: Springer;.[CrossRef]
    [Google Scholar]
  25. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  26. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  27. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  28. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  29. Ten L. N. , Jung H.-M. , Im W.-T. , Oh H. W. , Yang D.-C. , Yoo S.-A. , Lee S.-T. . ( 2009; ). Dokdonella ginsengisoli sp. nov., isolated from soil from a ginseng field, and emended description of the genus Dokdonella . . Int J Syst Evol Microbiol 59:, 1947–1952. [CrossRef] [PubMed]
    [Google Scholar]
  30. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  31. Tindall B. J. . ( 1990; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  32. Tóth E. M. , Borsodi A. K. , Euzéby J. P. , Tindall B. J. , Márialigeti K. . ( 2007; ). Proposal to replace the illegitimate genus name Schineria Toth et al. 2001 with the genus name Ignatzschineria gen. nov. and to replace the illegitimate combination Schineria larvae Toth et al. 2001 with Ignatzschineria larvae comb. nov.. Int J Syst Evol Microbiol 57:, 179–180. [CrossRef] [PubMed]
    [Google Scholar]
  33. Tóth E. M. , Schumann P. , Borsodi A. K. , Kéki Z. , Kovács A. L. , Márialigeti K. . ( 2008; ). Wohlfahrtiimonas chitiniclastica gen. nov., sp. nov., a new gammaproteobacterium isolated from Wohlfahrtia magnifica (Diptera: Sarcophagidae). . Int J Syst Evol Microbiol 58:, 976–981. [CrossRef] [PubMed]
    [Google Scholar]
  34. Ventosa A. , Marquez M. C. , Kocur M. , Tindall B. J. . ( 1993; ). Comparative study of “Micrococcus sp.” strains CCM 168 and CCM 1405 and members of the genus Salinicoccus . . Int J Syst Bacteriol 43:, 245–248. [CrossRef] [PubMed]
    [Google Scholar]
  35. Weon H.-Y. , Yoo S.-H. , Kim Y.-J. , Lee C.-M. , Kim B.-Y. , Jeon Y.-A. , Hong S.-B. , Anandham R. , Kwon S.-W. . ( 2009; ). Rudaea cellulosilytica gen. nov., sp. nov., isolated from soil. . Int J Syst Evol Microbiol 59:, 2308–2312. [CrossRef] [PubMed]
    [Google Scholar]
  36. Xie C. H. , Yokota A. . ( 2003; ). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. . J Gen Appl Microbiol 49:, 345–349. [CrossRef] [PubMed]
    [Google Scholar]
  37. Yoon J.-H. , Kang S.-J. , Oh T.-K. . ( 2006; ). Dokdonella koreensis gen. nov., sp. nov., isolated from soil. . Int J Syst Evol Microbiol 56:, 145–150. [CrossRef] [PubMed]
    [Google Scholar]
  38. Young C. C. , Kämpfer P. , Ho M. J. , Busse H. J. , Huber B. E. , Arun A. B. , Shen F. T. , Lai W. A. , Rekha P. D. . ( 2007; ). Arenimonas malthae sp. nov., a gammaproteobacterium isolated from an oil-contaminated site. . Int J Syst Evol Microbiol 57:, 2790–2793. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.034132-0
Loading
/content/journal/ijsem/10.1099/ijs.0.034132-0
Loading

Data & Media loading...

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error