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Abstract

A Gram-stain-negative, aerobic, motile, orange-pigmented, slightly halophilic, rod-shaped bacterium, designated strain JAMH 0132, was isolated from the trophosome of a tubeworm in Kagoshima Bay, Japan, and its taxonomic position was investigated using a polyphasic approach. The novel strain grew optimally at 28–30 °C and with about 2.0 % (w/v) NaCl. Chemotaxonomic analysis showed that Q-10 was the predominant respiratory quinone and that Cω7, C 2-OH and C were the major fatty acids. Sphingoglycolipid, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine were the major polar lipids. The genomic DNA G+C content was 60.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JAMH 0132 belonged to the family , within the class . The novel strain appeared most closely related to SW-150 (95.1 % 16S rRNA gene sequence similarity) and showed less sequence similarity with representatives of the genera , , and (<94.8 %). In having no detectable polyamine, strain JAMH 0132 differed from members of all genera currently in the family . On the basis of its phenotypic properties and phylogenetic distinctiveness, strain JAMH 0132 represents a novel species of a new genus in the family for which the name gen. nov., sp. nov. is proposed. The type strain of gen. nov., sp. nov. is JAMH 0132 ( = JCM 15549  = NCIMB 14486).

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2012-09-01
2021-10-16
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