1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 62, Issue Pt_9

gen. nov., sp. nov., isolated from a marine annelid worm Free

Abstract

A Gram-stain-negative, aerobic, motile, orange-pigmented, slightly halophilic, rod-shaped bacterium, designated strain JAMH 0132, was isolated from the trophosome of a tubeworm in Kagoshima Bay, Japan, and its taxonomic position was investigated using a polyphasic approach. The novel strain grew optimally at 28–30 °C and with about 2.0 % (w/v) NaCl. Chemotaxonomic analysis showed that Q-10 was the predominant respiratory quinone and that Cω7, C 2-OH and C were the major fatty acids. Sphingoglycolipid, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine were the major polar lipids. The genomic DNA G+C content was 60.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JAMH 0132 belonged to the family , within the class . The novel strain appeared most closely related to SW-150 (95.1 % 16S rRNA gene sequence similarity) and showed less sequence similarity with representatives of the genera , , and (<94.8 %). In having no detectable polyamine, strain JAMH 0132 differed from members of all genera currently in the family . On the basis of its phenotypic properties and phylogenetic distinctiveness, strain JAMH 0132 represents a novel species of a new genus in the family for which the name gen. nov., sp. nov. is proposed. The type strain of gen. nov., sp. nov. is JAMH 0132 ( = JCM 15549  = NCIMB 14486).

  • Published Online:
© 2012 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.034033-0
2012-09-01
2024-10-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/9/2224.html?itemId=/content/journal/ijsem/10.1099/ijs.0.034033-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A. 1993 Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press; [View Article]
     [Google Scholar]
  2. Baumann L., Baumann P., Mandel M., Allen R. D. 1972; Taxonomy of aerobic marine eubacteria. J Bacteriol 110:402–429[PubMed]
     [Google Scholar]
  3. Busse H.-J., Denner E. B. M., Buczolits S., Salkinoja-Salonen M., Bennasar A., Kämpfer P. 2003; Sphingomonas aurantiaca sp. nov., Sphingomonas aerolata sp. nov. and Sphingomonas faeni sp. nov., air- and dustborne and Antarctic, orange-pigmented, psychrotolerant bacteria, and emended description of the genus Sphingomonas . Int J Syst Evol Microbiol 53:1253–1260 [View Article][PubMed]
     [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
     [Google Scholar]
  5. Felsenstein J. 2009; phylip (phylogeny inference package), version 3.6a. http://evolution.genetics.washington.edu/phylip.html
  6. Fujii K., Satomi M., Morita N., Motomura T., Tanaka T., Kikuchi S. 2003; Novosphingobium tardaugens sp. nov., an oestradiol-degrading bacterium isolated from activated sludge of a sewage treatment plant in Tokyo. Int J Syst Evol Microbiol 53:47–52 [View Article][PubMed]
     [Google Scholar]
  7. Godoy F., Vancanneyt M., Martínez M., Steinbüchel A., Swings J., Rehm B. H. A. 2003; Sphingopyxis chilensis sp. nov., a chlorophenol-degrading bacterium that accumulates polyhydroxyalkanoate, and transfer of Sphingomonas alaskensis to Sphingopyxis alaskensis comb. nov.. Int J Syst Evol Microbiol 53:473–477 [View Article][PubMed]
     [Google Scholar]
  8. Hamana K., Takeuchi M. 1998; Polyamine profiles as chemotaxonomic marker within alpha, beta, gamma, delta, and epsilon subclasses of class Proteobacteria: distribution of 2-hydroxyputrescine and homospermidine. Microbiol Cult Collect 14:1–14
     [Google Scholar]
  9. Hamana K., Sakane T., Yokota A. 1994; A polyamine analysis of the genera Aquaspirillum, Magnetospirillum, Oceanospirillum and Spirillum . J Gen Appl Microbiol 40:75–82 [View Article]
     [Google Scholar]
  10. Hosoya R., Hamana K. 2003; Absence of cellular triamines in four novel flavobacteria located in Flavobacterium–Flexibacter–Cytophaga complex. Ann Gunma Health Sci 24:13–16
     [Google Scholar]
  11. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol 66:24–26[PubMed]
     [Google Scholar]
  12. Kämpfer P., Witzenberger R., Denner E. B. M., Busse H.-J., Neef A. 2002; Sphingopyxis witflariensis sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 52:2029–2034 [View Article][PubMed]
     [Google Scholar]
  13. Kim B.-S., Lim Y. W., Chun J. 2008; Sphingopyxis marina sp. nov. and Sphingopyxis litoris sp. nov., isolated from seawater. Int J Syst Evol Microbiol 58:2415–2419 [View Article][PubMed]
     [Google Scholar]
  14. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
     [Google Scholar]
  15. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
     [Google Scholar]
  16. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
     [Google Scholar]
  17. Maruyama T., Park H. D., Ozawa K., Tanaka Y., Sumino T., Hamana K., Hiraishi A., Kato K. 2006; Sphingosinicella microcystinivorans gen. nov., sp. nov., a microcystin-degrading bacterium. Int J Syst Evol Microbiol 56:85–89 [View Article][PubMed]
     [Google Scholar]
  18. MIDI 1999 Sherlock, Microbial Identification System, Operating Manual, version 3.0 Newark, DE: MIDI Inc;
     [Google Scholar]
  19. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  20. Miyazaki M., Nogi Y., Usami R., Horikoshi K. 2006; Shewanella surugensis sp. nov., Shewanella kaireitica sp. nov. and Shewanella abyssi sp. nov., isolated from deep-sea sediments of Suruga Bay, Japan. Int J Syst Evol Microbiol 56:1607–1613 [View Article][PubMed]
     [Google Scholar]
  21. Miyazaki M., Nogi Y., Ohta Y., Hatada Y., Fujiwara Y., Ito S., Horikoshi K. 2008; Microbulbifer agarilyticus sp. nov. and Microbulbifer thermotolerans sp. nov., agar-degrading bacteria isolated from deep-sea sediment. Int J Syst Evol Microbiol 58:1128–1133 [View Article][PubMed]
     [Google Scholar]
  22. Saito H., Miura K. I. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619–629 [View Article][PubMed]
     [Google Scholar]
  23. Takeuchi M., Hamana K., Hiraishi A. 2001; Proposal of the genus Sphingomonas sensu stricto and three new genera, Sphingobium, Novosphingobium and Sphingopyxis, on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 51:1405–1417[PubMed]
     [Google Scholar]
  24. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
     [Google Scholar]
  25. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  26. Ushiba Y., Takahara Y., Ohta H. 2003; Sphingobium amiense sp. nov., a novel nonylphenol-degrading bacterium isolated from a river sediment. Int J Syst Evol Microbiol 53:2045–2048 [View Article][PubMed]
     [Google Scholar]
  27. Yabuuchi E., Kosako Y., Fujiwara N., Naka T., Matsunaga I., Ogura H., Kobayashi K. 2002; Emendation of the genus Sphingomonas Yabuuchi et al. 1990 and junior objective synonymy of the species of three genera, Sphingobium, Novosphingobium and Sphingopyxis, in conjunction with Blastomonas ursincola . Int J Syst Evol Microbiol 52:1485–1496 [View Article][PubMed]
     [Google Scholar]
  28. Yoon J.-H., Oh T.-K. 2005; Sphingopyxis flavimaris sp. nov., isolated from sea water of the Yellow Sea in Korea. Int J Syst Evol Microbiol 55:369–373 [View Article][PubMed]
     [Google Scholar]
  29. Yoon J.-H., Lee C.-H., Yeo S.-H., Oh T.-K. 2005; Sphingopyxis baekryungensis sp. nov., an orange-pigmented bacterium isolated from sea water of the Yellow Sea in Korea. Int J Syst Evol Microbiol 55:1223–1227 [View Article][PubMed]
     [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.034033-0
Loading
Parasphingopyxis lamellibrachiae gen. nov., sp. nov., isolated from a marine annelid worm
Int J Syst Evol Microbiol 62, 2224 (2012); https://doi.org/10.1099/ijs.0.034033-0
/content/journal/ijsem/10.1099/ijs.0.034033-0
/content/journal/ijsem/10.1099/ijs.0.034033-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error