1887

Abstract

A novel pale-pink-coloured strain, designated R9-65, was isolated from a tundra soil near Ny-Ålesund, Svalbard Archipelago, Norway (78° N). The cells were facultatively anaerobic, Gram-staining-negative, non-motile and rod-shaped. Growth occurred at 4–32 °C (optimum, 25–28 °C), at pH 5.0–9.0 (optimum, pH 6.0–7.0) and with 0–1.0 % (w/v) NaCl (optimum, no NaCl). Flexirubin-type pigments were absent. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R9-65 belonged to the genus in the family . The 16S rRNA gene sequence similarity between strain R9-65 and type strains of related species ranged from 93.4 to 96.6 %. Strain R9-65 contained summed feature 3 (Cω7 and/or Cω6, 34.3 %) and iso-C (20.3 %) as major cellular fatty acids, MK-7 as the major respiratory quinone, and phosphatidylethanolamine as the main polar lipid. The DNA G+C content of strain R9-65 was 47.2 mol%. On the basis of phylogenetic, physiological and chemotaxonomic data, strain R9-65 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R9-65 ( = CCTCC AB 2010331 = NRRL B-59458).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.033902-0
2012-07-01
2020-01-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/7/1630.html?itemId=/content/journal/ijsem/10.1099/ijs.0.033902-0&mimeType=html&fmt=ahah

References

  1. An D.-S., Yin C.-R., Lee S.-T., Cho C.-H.. ( 2009;). Mucilaginibacter daejeonensis sp. nov., isolated from dried rice straw. . Int J Syst Evol Microbiol 59:, 1122–1125. [CrossRef][PubMed]
    [Google Scholar]
  2. Baik K. S., Park S. C., Kim E. M., Lim C. H., Seong C. N.. ( 2010;). Mucilaginibacter rigui sp. nov., isolated from wetland freshwater, and emended description of the genus Mucilaginibacter. . Int J Syst Evol Microbiol 60:, 134–139. [CrossRef][PubMed]
    [Google Scholar]
  3. Barrow G. I., Feltham R. K. A.. (editors) ( 1993;). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B..for the Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  5. Bowman J. P.. ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  6. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W.. ( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef][PubMed]
    [Google Scholar]
  7. Doetsch R. N.. ( 1981;). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology, pp. 21–33. Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. H... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  8. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  9. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  10. Felsenstein J.. ( 2005;). phylip (Phylogeny Inference Package), Version 3.6. Distributed by the author, Department of Genome Sciences, University of Washington, Seattle..
    [Google Scholar]
  11. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  12. Jeon Y., Lee S.-S., Chung B. S., Kim J. M., Bae J.-W., Park S. K., Jeon C. O.. ( 2009;). Mucilaginibacter oryzae sp. nov., isolated from soil of a rice paddy. . Int J Syst Evol Microbiol 59:, 1451–1454. [CrossRef][PubMed]
    [Google Scholar]
  13. Joung Y., Joh K.. ( 2011;). Mucilaginibacter myungsuensis sp. nov., isolated from mesotrophic artificial lake in Korea. . Int J Syst Evol Microbiol 61:, 1506–1510. [CrossRef][PubMed]
    [Google Scholar]
  14. Kang S. J., Jung Y. T., Oh K. H., Oh T. K., Yoon J. H.. ( 2011;). Mucilaginibacter boryungensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 61:, 1549–1553. [CrossRef][PubMed]
    [Google Scholar]
  15. Kato M., Muto Y., Tanaka-Bandoh K., Watanabe K., Ueno K.. ( 1995;). Sphingolipid composition in Bacteroides species. . Anaerobe 1:, 135–139. [CrossRef][PubMed]
    [Google Scholar]
  16. Kim B. C., Lee K. H., Kim M. N., Lee J., Shin K. S.. ( 2010;). Mucilaginibacter dorajii sp. nov., isolated from the rhizosphere of Platycodon grandiflorum. . FEMS Microbiol Lett 309:, 130–135.[PubMed]
    [Google Scholar]
  17. Kim B. C., Poo H., Lee K. H., Kim M. N., Kwon O.-Y., Shin K. S.. ( 2012;). Mucilaginibacter angelicae sp. nov., a novel bacterium isolated from Angelica polymorpha Maxim. . Int J Syst Evol Microbiol 62:, 55–60. [CrossRef][PubMed]
    [Google Scholar]
  18. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  19. Lane D. J.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–147. Edited by Stackebrandt E., Goodfellow M... Chichester:: Wiley;.
    [Google Scholar]
  20. Luo X., Zhang L., Dai J., Liu M., Zhang K., An H., Fang C.. ( 2009;). Mucilaginibacter ximonensis sp. nov., isolated from Tibetan soil. . Int J Syst Evol Microbiol 59:, 1447–1450. [CrossRef][PubMed]
    [Google Scholar]
  21. Madhaiyan M., Poonguzhali S., Lee J. S., Senthilkumar M., Lee K. C., Sundaram S.. ( 2010;). Mucilaginibacter gossypii sp. nov. and Mucilaginibacter gossypiicola sp. nov., plant-growth-promoting bacteria isolated from cotton rhizosphere soils. . Int J Syst Evol Microbiol 60:, 2451–2457. [CrossRef][PubMed]
    [Google Scholar]
  22. Männistö M. K., Tiirola M., McConnell J., Häggblom M. M.. ( 2010;). Mucilaginibacter frigoritolerans sp. nov., Mucilaginibacter lappiensis sp. nov. and Mucilaginibacter mallensis sp. nov., isolated from soil and lichen samples. . Int J Syst Evol Microbiol 60:, 2849–2856. [CrossRef][PubMed]
    [Google Scholar]
  23. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  24. Pankratov T. A., Tindall B. J., Liesack W., Dedysh S. N.. ( 2007;). Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog. . Int J Syst Evol Microbiol 57:, 2349–2354. [CrossRef][PubMed]
    [Google Scholar]
  25. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  26. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  27. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  28. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  29. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). MEGA4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  30. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  31. Tindall B. J.. ( 1990;). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  32. Urai M., Aizawa T., Nakagawa Y., Nakajima M., Sunairi M.. ( 2008;). Mucilaginibacter kameinonensis sp., nov., isolated from garden soil. . Int J Syst Evol Microbiol 58:, 2046–2050. [CrossRef][PubMed]
    [Google Scholar]
  33. Xie C. H., Yokota A.. ( 2003;). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. . J Gen Appl Microbiol 49:, 345–349. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.033902-0
Loading
/content/journal/ijsem/10.1099/ijs.0.033902-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error