1887

Abstract

A Gram-positive, rod-shaped, endospore-forming bacterium, designated strain BLB-1, was isolated from samples of tidal flat sediment from the Yellow Sea. 16S rRNA gene sequence analysis demonstrated that the isolate belonged to the rRNA group 2 and was closely related to CIP 108446 (97.4 %), ATCC PTA-4993 (96.7 %), DSM 2898 (96.2 %) and DSM 17140 (95.9 %). Sequence similarities with related species in other genera, including , and , were <96.1 %. Chemotaxonomic data supported the affiliation of strain BLB-1 with the genus . The major menaquinone was MK-7, the cell-wall sugars were glucose and xylose, the cell-wall peptidoglycan type was A4α (-Lys–-Asp), the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and several unknown phospholipids, and the major fatty acids were anteiso-C (35.6 %), iso-C (25.6 %) and anteiso-C (16.5 %). The most closely related species, and , were also assigned to this genus based on phylogenetic analysis and phenotypic data. The results of DNA–DNA hybridizations and phenotypic tests supported the differentiation of all three taxa from species of the genus with validly published names. Thus, strain BLB-1 ( = KCTC 13296  = JCM 15800) represents a novel species, for which the name sp. nov. is proposed. It is also proposed that CIP 108446 ( = 4400831 = CCUG49529  = KCTC 13178) and NBRC 100172 ( = 34hs-1  = ATCC PTA-4993  = NRRL B-30641  = DSM 18869  = CIP 108263  = KCTC 3961) be transferred to the genus as comb. nov. and comb. nov., respectively.

Funding
This study was supported by the:
  • Korea Science and Engineering Foundation (KOSEF) (Award M10508040002-07N0804-00216)
  • KRIBB Research Initiative Program
  • Ministry of Education, Science and Technology, Republic of Korea
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2012-10-01
2022-01-21
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