1887

Abstract

Gram-positive, rod-shaped bacteria, strains Hh8, Hh15 and Hh40-2, were isolated from the No. 1 glacier in Xinjiang, north-west China. Colonies of strain Hh8 were orange–yellow, convex and round on PYG plates. Strain Hh8 grew at 0–19 °C and pH 5.5–10.5. Colonies of strain Hh15, which was able to grow at 0–20 °C and pH 5.5–12, were lemon yellow, convex and round on PYG plates. Phylogenetic analysis based on 16S rRNA gene sequences showed that these three strains were related to members of the genus . The major cellular fatty acids of the novel strains were anteiso-C, iso-C, iso-C and anteiso-C A. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, two novel species, sp. nov. (type strain Hh8  = CGMCC 1.11215  = NBRC 107879) and sp. nov. (type strain Hh15  = CGMCC 1.11210  = NBRC 107880), are proposed.

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2012-06-01
2020-01-19
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References

  1. Dong X.-Z., Cai M.-Y.. (editors) ( 2001;). Determination of biochemical properties. . In Manual for the Systematic Identification of General Bacteria, pp. 370–398. Beijing:: Science Press;.
    [Google Scholar]
  2. Bajerski F., Ganzert L., Mangelsdorf K., Lipski A., Wagner D.. ( 2011;). Cryobacterium arcticum sp. nov., a psychrotolerant bacterium from an Arctic soil. . Int J Syst Evol Microbiol 61:, 1849–1853. [CrossRef][PubMed]
    [Google Scholar]
  3. Collins M. D.. ( 1985;). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E... London:: Academic Press;.
    [Google Scholar]
  4. Dastager S. G., Lee J.-C., Ju Y.-J., Park D. J., Kim C.-J.. ( 2008;). Cryobacterium mesophilum sp. nov., a novel mesophilic bacterium. . Int J Syst Evol Microbiol 58:, 1241–1244. [CrossRef][PubMed]
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A.. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef][PubMed]
    [Google Scholar]
  6. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  7. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  8. Inoue K., Komagata K.. ( 1976;). Taxonomic study on obligately psychrophilic bacteria isolated from Antarctica. . J Gen Appl Microbiol 22:, 165–176. [CrossRef]
    [Google Scholar]
  9. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  10. Marmur J., Doty P.. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef][PubMed]
    [Google Scholar]
  11. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M.. ( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27:, 104–117. [CrossRef]
    [Google Scholar]
  12. Reddy G. S. N., Pradhan S., Manorama R., Shivaji S.. ( 2010;). Cryobacterium roopkundense sp. nov., a psychrophilic bacterium isolated from glacial soil. . Int J Syst Evol Microbiol 60:, 866–870. [CrossRef][PubMed]
    [Google Scholar]
  13. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  14. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  15. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  16. Suzuki K., Sasaki J., Uramoto M., Nakase T., Komagata K.. ( 1997;). Cryobacterium psychrophilum gen. nov., sp. nov., nom. rev., comb. nov., an obligately psychrophilic actinomycete to accommodate “Curtobacterium psychrophilum” Inoue and Komagata 1976. . Int J Syst Bacteriol 47:, 474–478. [CrossRef][PubMed]
    [Google Scholar]
  17. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4. 0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  18. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef][PubMed]
    [Google Scholar]
  19. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  20. Wu C., Lu X., Qin M., Wang Y., Ruan J.. ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16:, 176–178.
    [Google Scholar]
  21. Zhang D.-C., Wang H.-X., Cui H.-L., Yang Y., Liu H.-C., Dong X.-Z., Zhou P.-J.. ( 2007;). Cryobacterium psychrotolerans sp. nov., a novel psychrotolerant bacterium isolated from the China No. 1 glacier. . Int J Syst Evol Microbiol 57:, 866–869. [CrossRef][PubMed]
    [Google Scholar]
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