1887

Abstract

Two novel obligately anaerobic, Gram-stain-positive, saccharolytic and non-proteolytic spore-forming bacilli (strains CD3 : 22 and N1) are described. Strain CD3 : 22 was isolated from a biopsy of the small intestine of a child with coeliac disease, and strain N1 from the saliva of a healthy young man. The cells of both strains were observed to be filamentous, approximately 5 to >20 µm long, some of them curving and with swellings. The novel organisms produced HS, NH, butyric acid and acetic acid as major metabolic end products. Phylogenetic analyses, based on comparative 16S rRNA gene sequencing, revealed close relationships (98 % sequence similarity) between the two isolates, as well as the type strain of and four other bacterium-/ -like organisms. This group of bacteria were clearly different from any of the 19 known genera in the family . While species are reported to be non-spore-forming, reanalysis of CCUG 28089 confirmed that the bacterium is indeed able to form spores. Based on 16S rRNA gene sequencing, phenotypic and biochemical properties, strains CD3 : 22 and N1 represent novel species of a new and distinct genus, named gen. nov., in the family [within the order , class , phylum ]. Strain CD3 : 22 ( = CCUG 58757  = DSM 23576) is the type strain of the type species, gen. nov., sp. nov., of the proposed new genus. Strain N1 ( = CCUG 60305 = DSM 24553) is the type strain of sp. nov. Moreover, is reclassified as comb. nov. (type strain CCUG 28089  = ATCC 33271  = CIP 105341  = DSM 3986  = JCM 11021  = VPI 11763).

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2012-11-01
2019-12-06
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