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Abstract

Two yellow, rod-shaped, Gram-reaction-negative, strictly aerobic bacterial strains, designated JC2682 and JC2681, were isolated from a seawater sample of Jeju Island, Korea. The isolates required sea salts for growth and showed gliding motility. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strains JC2682 and JC2681 shared 96.9 % similarity and formed a robust monophyletic group within the radiation encompassing the family This distinct clade, which had varying branch positions depending on the tree-making algorithm used, represents a new genus within the family . The predominant isoprenoid quinone (menaquinone 6, MK-6) and DNA G+C content (27–33 mol%) were consistent with its assignment to the family , but the polar lipid profile, fatty acid composition and overall phenotypic traits demonstrated that the test strains were not closely affiliated to any recognized genera. Based on data from the present polyphasic taxonomic study, strains JC2682 and JC2681 are considered to represent two novel species of a new genus in the family , for which the names gen. nov., sp. nov. and sp. nov. are proposed, respectively. The type strain of is JC2682 ( = KACC 14158  = JCM 17113). The type strain of is JC2681 ( = KACC 14157  = JCM 17112). Amended descriptions of the genera and are provided.

Funding
This study was supported by the:
  • Priority Research Centers Program (Award #2 00 8- 00 5-J 00 201)
  • National Research Foundation (Award #2010-0017955)
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2012-05-01
2021-08-04
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References

  1. Bernardet J. F., Nakagawa Y., Holmes B. for the Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  2. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:1861–1868[PubMed]
    [Google Scholar]
  3. Bowman J. P., Nichols D. S. 2005; Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp. nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia, Gelidibacter and Gillisia . Int J Syst Evol Microbiol 55:1471–1486 [View Article][PubMed]
    [Google Scholar]
  4. Bowman J. P., McCammon S. A., Brown J. L., Nichols P. D., McMeekin T. A. 1997; Psychroserpens burtonensis gen. nov., sp. nov., and Gelidibacter algens gen. nov., sp. nov., psychrophilic bacteria isolated from Antarctic lacustrine and sea ice habitats. Int J Syst Bacteriol 47:670–677 [View Article][PubMed]
    [Google Scholar]
  5. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [View Article][PubMed]
    [Google Scholar]
  6. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  7. Collins M. D. 1985; Analysis of isoprenoid quinones. Methods Microbiol 18:329–366 [View Article]
    [Google Scholar]
  8. Embley T. M., Wait R. 1994; Structural lipids of eubacteria. In Chemical Methods in Prokaryotic Systematics pp. 121–161 Edited by Goodfellow M., O’Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  9. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  10. Felsenstein J. 1993; phylip (phylogeny inference package) version 3.5.1. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
    [Google Scholar]
  11. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24:54–63 [View Article]
    [Google Scholar]
  12. Jeon Y. S., Chung H., Park S., Hur I., Lee J. H., Chun J. 2005; jphydit: a java-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21:3171–3173 [View Article][PubMed]
    [Google Scholar]
  13. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp. 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  14. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [View Article][PubMed]
    [Google Scholar]
  15. Kwon K. K., Lee S. J., Park J. H., Ahn T. Y., Lee H. K. 2006; Psychroserpens mesophilus sp. nov., a mesophilic marine bacterium belonging to the family Flavobacteriaceae isolated from a young biofilm. Int J Syst Evol Microbiol 56:1055–1058 [View Article][PubMed]
    [Google Scholar]
  16. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
    [Google Scholar]
  17. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athayle M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  18. Nedashkovskaya O. I., Kim S. B., Han S. K., Rhee M. S., Lysenko A. M., Rohde M., Zhukova N. V., Frolova G. M., Mikhailov V. V., Bae K. S. 2004; Algibacter lectus gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from green algae. Int J Syst Evol Microbiol 54:1257–1261 [View Article][PubMed]
    [Google Scholar]
  19. Nedashkovskaya O. I., Vancanneyt M., Kim S. B., Hoste B., Bae K. S. 2007; Algibacter mikhailovii sp. nov., a novel marine bacterium of the family Flavobacteriaceae, and emended description of the genus Algibacter . Int J Syst Evol Microbiol 57:2147–2150 [View Article][PubMed]
    [Google Scholar]
  20. Nedashkovskaya O. I., Kwon K. K., Yang S. H., Lee H. S., Chung K. H., Kim S. J. 2008; Lacinutrix algicola sp. nov. and Lacinutrix mariniflava sp. nov., two novel marine alga-associated bacteria and emended description of the genus Lacinutrix . Int J Syst Evol Microbiol 58:2694–2698 [View Article][PubMed]
    [Google Scholar]
  21. Ronquist F., Huelsenbeck J. P. 2003; MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574 [View Article][PubMed]
    [Google Scholar]
  22. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  23. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  24. Swofford D. L. 1998; Phylogenetic analysis using parsimony (paup), version 4. Sunderland, MA: Sinauer Associates..
    [Google Scholar]
  25. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  26. Weeks O. B. 1981; Preliminary studies of the pigments of Flavobacterium breve NCTC 11099 and Flavobacterium odoratum NCTC 11036. In The Flavobacterium-Cytophaga Group pp. 108–114 Edited by Reichenbach H., Weeks O. B. Weinheim: Gesellschaft für Biotechnologische Forschung;
    [Google Scholar]
  27. Woese C. R., Magrum L. J., Gupta R., Siegel R. B., Stahl D. A., Kop J., Crawford N., Brosius J., Gutell R. other authors 1980; Secondary structure model for bacterial 16S ribosomal RNA: phylogenetic, enzymatic and chemical evidence. Nucleic Acids Res 8:2275–2294 [View Article][PubMed]
    [Google Scholar]
  28. ZoBell C. E. 1941; Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4:42–75
    [Google Scholar]
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