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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 62, Issue Pt_8

sp. nov., a cellulase-producing bacterium isolated from mangrove soil Free

Abstract

A taxonomic study was carried out on a cellulase-producing bacterium, strain G21, isolated from mangrove soil in Xiamen, Fujian province, China. Cells were Gram-negative, slightly curved rods, motile with a single polar flagellum. The strain grew at 15–40 °C and in 0.5–10 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain G21 belonged to the genus and formed a clade with ATCC 350116 (97.4 % sequence similarity), LMG 7894 (97.1 %) and CECT 5869 (96.1 %). However, multilocus sequence analysis (using , , , , and sequences) and DNA–DNA hybridization experiments indicated that the strain was distinct from the closest related species. Additionally, strain G21 could be differentiated from them phenotypically by the ability to grow in 10 % NaCl but not on TCBS plates, its enzyme activity spectrum, citrate utilization, oxidization of various carbon sources, hydrolysis of several substrates and its cellular fatty acid profile. The G+C content of the genomic DNA was 46.0 mol%. The major cellular fatty acids were summed feature 3 (Cω7 and/or iso-C 2-OH), C and Cω7. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol, with trace amounts of diphosphatidylglycerol. The predominant quinones were Q-8 and Q-7. Based on phylogenetic, phenotypic and chemotaxonomic characteristics and DNA–DNA hybridization analysis, it is concluded that strain G21 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is G21 ( = DSM 22851  = CGMCC 1.10228).

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Funding
This study was supported by the:
  • Third Institute of Oceanography, State Oceanic Administration, China
  • Marine Scientific Research Special Foundation for Public Sector Program (Award 200805050)
© 2012 IUMS
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2012-08-01
2025-03-23
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References

  1. Baumann P., Furniss A. L., Lee J. V. 1984; Genus I. Vibrio Pacini 1854. In Bergey's Manual of Systematic Bacteriology pp. 518–538 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
     [Google Scholar]
  2. Beaz-Hidalgo R., Cleenwerck I., Balboa S., Prado S., De Vos P., Romalde J. L. 2009; Vibrio breoganii sp. nov., a non-motile, alginolytic, marine bacterium within the Vibrio halioticoli clade. Int J Syst Evol Microbiol 59:1589–1594 [View Article][PubMed]
     [Google Scholar]
  3. Bleicher A., Neuhaus K., Scherer S. 2010; Vibrio casei sp. nov., isolated from the surfaces of two French red smear soft cheeses. Int J Syst Evol Microbiol 60:1745–1749 [View Article][PubMed]
     [Google Scholar]
  4. Chang H. W., Roh S. W., Kim K. H., Nam Y. D., Jeon C. O., Oh H. M., Bae J. W. 2008; Vibrio areninigrae sp. nov., a marine bacterium isolated from black sand. Int J Syst Evol Microbiol 58:1903–1906 [View Article][PubMed]
     [Google Scholar]
  5. Chimetto L. A., Cleenwerck I., Alves N. Jr, Silva B. S., Brocchi M., Willems A., De Vos P., Thompson F. L. 2011a; Vibrio communis sp. nov., isolated from the marine animals Mussismilia hispida, Phyllogorgia dilatata, Palythoa caribaeorum, Palythoa variabilis and Litopenaeus vannamei . Int J Syst Evol Microbiol 61:362–368 [View Article][PubMed]
     [Google Scholar]
  6. Chimetto L., Cleenwerck I., Moreira A. P. B., Brocchi M., Willems A., De Vos P., Thompson F. L. 2011b; Vibrio variabilis sp. nov. and Vibrio marinus sp. nov., isolated from Palythoa caribaeorum . Int J Syst Evol Microbiol 61:3009–3015 [View Article][PubMed]
     [Google Scholar]
  7. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
     [Google Scholar]
  8. Coram N. J., Rawlings D. E. 2002; Molecular relationship between two groups of the genus Leptospirillum and the finding that Leptospirillum ferriphilum sp. nov. dominates South African commercial biooxidation tanks that operate at 40 °C. Appl Environ Microbiol 68:838–845 [View Article][PubMed]
     [Google Scholar]
  9. Diéguez A. L., Beaz-Hidalgo R., Cleenwerck I., Balboa S., de Vos P., Romalde J. L. 2011; Vibrio atlanticus sp. nov. and Vibrio artabrorum sp. nov., isolated from the clams Ruditapes philippinarum and Ruditapes decussatus . Int J Syst Evol Microbiol 61:2406–2411 [View Article][PubMed]
     [Google Scholar]
  10. Dong X.-Z., Cai M.-Y. (editors) 2001; Determination of biochemical properties. In Manual for the Systematic Identification of General Bacteria pp. 370–398 Beijing: Science Press; (in Chinese)
    [Google Scholar]
  11. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
     [Google Scholar]
  12. Gao Z. M., Ruan L. W., Chen X. L., Zhang Y. Z., Xu X. 2010; A novel salt-tolerant endo-beta-1,4-glucanase Cel5A in Vibrio sp. G21 isolated from mangrove soil. Appl Microbiol Biotechnol 87:1373–1382 [View Article][PubMed]
     [Google Scholar]
  13. Gomez-Gil B., Fajer-Avila E. F., Pascual J., Macián M. C., Pujalte M. J., Garay E., Roque A. 2008; Vibrio sinaloensis sp. nov., isolated from the spotted rose snapper, Lutjanus guttatus Steindachner, 1869. Int J Syst Evol Microbiol 58:1621–1624 [View Article][PubMed]
     [Google Scholar]
  14. Hoffmann M., Monday S. R., Allard M. W., Strain E. A., Whittaker P., Naum M., McCarthy P. J., Lopez J. V., Fischer M., Brown E. W. 2012; Vibrio caribbeanicus sp. nov., isolated from marine sponge Scleritoderma cyanea . Int J Syst Evol Microbiol 62:1736–1743 [View Article][PubMed]
     [Google Scholar]
  15. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
     [Google Scholar]
  16. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
     [Google Scholar]
  17. Liu C., Shao Z. 2005; Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55:1181–1186 [View Article][PubMed]
     [Google Scholar]
  18. Macián M. C., Garay E., Grimont P. A., Pujalte M. J. 2004; Vibrio ponticus sp. nov., a neighbour of V. fluvialis-V. furnissii clade, isolated from gilthead sea bream, mussels and seawater. Syst Appl Microbiol 27:535–540 [View Article][PubMed]
     [Google Scholar]
  19. Mesbah M., Whitman W. B. 1989; Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr A 479:297–306 [View Article][PubMed]
     [Google Scholar]
  20. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586[PubMed]
     [Google Scholar]
  21. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  22. Rameshkumar N., Fukui Y., Sawabe T., Nair S. 2008; Vibrio porteresiae sp. nov., a diazotrophic bacterium isolated from a mangrove-associated wild rice (Porteresia coarctata Tateoka). Int J Syst Evol Microbiol 58:1608–1615 [View Article][PubMed]
     [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  24. Sasser M. 1997; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.;
  25. Sawabe T., Kita-Tsukamoto K., Thompson F. L. 2007; Inferring the evolutionary history of vibrios by means of multilocus sequence analysis. J Bacteriol 189:7932–7936 [View Article][PubMed]
     [Google Scholar]
  26. Sheu S. Y., Jiang S. R., Chen C. A., Wang J. T., Chen W. M. 2011; Vibrio stylophorae sp. nov., isolated from the reef-building coral Stylophora pistillata . Int J Syst Evol Microbiol 61:2180–2185 [View Article][PubMed]
     [Google Scholar]
  27. Syn C. K., Swarup S. 2000; A scalable protocol for the isolation of large-sized genomic DNA within an hour from several bacteria. Anal Biochem 278:86–90 [View Article][PubMed]
     [Google Scholar]
  28. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  29. Teather R. M., Wood P. J. 1982; Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 43:777–780[PubMed]
     [Google Scholar]
  30. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
     [Google Scholar]
  31. Thompson F. L., Iida T., Swings J. 2004; Biodiversity of vibrios. Microbiol Mol Biol Rev 68:403–431 [View Article][PubMed]
     [Google Scholar]
  32. Thompson F. L., Gevers D., Thompson C. C., Dawyndt P., Naser S., Hoste B., Munn C. B., Swings J. 2005; Phylogeny and molecular identification of vibrios on the basis of multilocus sequence analysis. Appl Environ Microbiol 71:5107–5115 [View Article][PubMed]
     [Google Scholar]
  33. Wang Y., Zhang X. H., Yu M., Wang H., Austin B. 2010; Vibrio atypicus sp. nov., isolated from the digestive tract of the Chinese prawn (Penaeus chinensis O’sbeck). Int J Syst Evol Microbiol 60:2517–2523 [View Article][PubMed]
     [Google Scholar]
  34. Wang H., Liu J., Wang Y., Zhang X. H. 2011; Vibrio marisflavi sp. nov., isolated from seawater. Int J Syst Evol Microbiol 61:568–573 [View Article][PubMed]
     [Google Scholar]
  35. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
     [Google Scholar]
  36. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703[PubMed]
     [Google Scholar]
  37. Wilkinson S. G. 1988; Gram-negative bacteria. In Microbial Lipids vol. 1 pp. 299–488 Edited by Ratledge C., Wilkinson S. G. New York: Academic Press;
     [Google Scholar]
  38. Xu X. W., Wu Y. H., Wang C. S., Oren A., Wu M. 2009; Vibrio hangzhouensis sp. nov., isolated from sediment of the East China Sea. Int J Syst Evol Microbiol 59:2099–2103 [View Article][PubMed]
     [Google Scholar]
  39. Yoshizawa S., Wada M., Kita-Tsukamoto K., Ikemoto E., Yokota A., Kogure K. 2009; Vibrio azureus sp. nov., a luminous marine bacterium isolated from seawater. Int J Syst Evol Microbiol 59:1645–1649 [View Article][PubMed]
     [Google Scholar]
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Vibrio xiamenensis sp. nov., a cellulase-producing bacterium isolated from mangrove soil
Int J Syst Evol Microbiol 62, 1958 (2012); https://doi.org/10.1099/ijs.0.033597-0
/content/journal/ijsem/10.1099/ijs.0.033597-0
/content/journal/ijsem/10.1099/ijs.0.033597-0
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