1887

Abstract

A heterotrophic, aerobic bacterium, designated strain SH6-1, was obtained from a seawater sample collected from the open North Sea during a phytoplankton bloom. Strain SH6-1 was isolated from a 10 dilution culture, which indicated a high abundance of this organism in the environmental sample. 16S rRNA gene sequence comparison revealed that strain SH6-1 belonged to the marine clade (order ) within the class . CL-ES2 was the closest phylogenetic neighbour (96.4 % 16S rRNA gene sequence similarity). Cells of strain SH6-1 were small or elongated irregular rods. Optimal growth occurred between 20 and 25 °C and between pH 7.5 and 9.0 with peptone and yeast extract. On marine agar, the isolate formed non-pigmented, small, circular, convex colonies. For growth, cells required sodium ions and the vitamins pantothenic acid and nicotinic acid amide. The DNA G+C content was 53.8 mol%. The fatty acids (>1 %) were C 3-OH, C, C, C 3-OH, C, Cω7, C and 11-methyl Cω7. The polar lipid pattern indicated the presence of phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid and one unidentified phospholipid. The major respiratory lipoquinone was ubiquinone Q-10. Strain SH6-1 contained the genes , which code for the bacterial photosynthesis reaction centre; however, no bacteriochlorophyll could be detected. Physiological, genotypic and phenotypic differences from support the description of a novel genus and species, for which we suggest the name gen. nov., sp. nov; the type strain of the type species is SH6-1 ( = DSM 23709 = LMG 25294).

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2012-07-01
2019-10-24
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