1887

Abstract

A bacterial strain (B2-7) capable of degrading a wide range of polycyclic aromatic hydrocarbon compounds (2–4 rings) was isolated from a water sample taken from Botan Oil Port in Xiamen, China. The isolate was Gram-negative, short-rod-shaped, aerobic, non-motile and formed yellow-pigmented colonies on LB medium. Cells of strain B2-7 were catalase-positive and oxidase-negative. Optimal growth of strain B2-7 was observed at pH 7.0, at 26 °C and in 0.5 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain B2-7 grouped with members of the genus and it showed 16S rRNA gene sequence similarity of 95.40 % to YIM 003. The major polar lipids were phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid. Q-10 and -homospermidine were the predominant ubiquinone and polyamine components, respectively. The major fatty acids were Cω7 (67.2 %), C 2-OH (10.0 %) and C (9.6 %). The G+C content of the genomic DNA was 61.8 mol%. Based on phenotypic properties, and phylogenetic and genomic data, strain B2-7 represents a novel species of the genus within the class , for which the name sp. nov. is proposed. The type strain is B2-7 ( = KCCM 42951 = JCM 16711).

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2012-06-01
2019-10-19
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