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Abstract

A Gram-stain-negative, aerobic, non-flagellated, non-gliding and short rod- or rod-shaped bacterial strain, designated BB-My20, was isolated from tidal flat sediment taken from the southern coast of Korea. Strain BB-My20 grew optimally at 37 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. A phylogenetic tree based on 16S rRNA gene sequences showed that strain BB-My20 fell within the clade comprising species, joining HY1, with which it had a 16S rRNA gene sequence similarity value of 97.4 %. It exhibited 95.4–96.9 % sequence similarity to the type strains of other members of the genus . Strain BB-My20 contained MK-6 as the predominant menaquinone and iso-C, anteiso-C and iso-C 3-OH as the major fatty acids. The major polar lipids detected in strain BB-My20 and JCM 14015 were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain BB-My20 was 45.1 mol% and its mean DNA–DNA relatedness value with JCM 14015 was 4.5 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain BB-My20 can be distinguished from the four recognized species of the genus . On the basis of the data presented, strain BB-My20 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is BB-My20 ( = KCTC 23579 = CCUG 60898).

Funding
This study was supported by the:
  • , Program for Collection, Management and Utilization of Biological Resources , (Award grant M10867010003)
  • , 21C Frontier Program of Microbial Genomics and Applications , (Award grant M10867010003)
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2012-05-01
2020-07-08
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