1887

Abstract

A Gram-positive, non-endospore-forming bacterium, designated strain SK 18, was isolated from surface soil of a landfill site by dilution plating on trypticase soy broth agar. Preliminary characterization of strain SK 18 via biochemical tests, analysis of fatty acid methyl esters and partial 16S rRNA gene sequencing placed it within the genus . Analysis of the cell wall indicated that the peptidoglycan was of cross-linkage type B, containing the amino acids lysine and ornithine and with muramic acid in the -glycolyl form. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid. The major fatty acids of the cell membrane were anteiso-C, anteiso-C and iso-C. These data further strengthened placement of the strain within the genus . Strain SK 18 shared highest 16S rRNA gene sequence similarity (97.2 %) with DSM 16931. Levels of similarity with the type strains of all other recognized species were less than 97.0 %. DNA–DNA hybridization experiments with strain SK 18 and its closest relative, DSM 16931, revealed a low reassociation value of 39.0 % (σ = 3.8 %). Moreover, strain SK 18 showed a number of differences in phenotypic characteristics (colony colour, catalase activity, hydrolysis of polymers, acid production from sugars and oxidation of various substrates), and its DNA G+C content was also higher than that of DSM 16931. These data indicated that strain SK 18 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SK 18 ( = MTCC 7185 = JCM 14034). An emended description of the genus is also provided.

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2012-09-01
2019-10-14
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