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Abstract

A Gram-stain-negative, aerobic, non-flagellated, non-spore-forming, motile (by gliding) bacterial strain, designated M-M6, was isolated from marine sand of Geoje island, Korea. Strain M-M6 grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain M-M6 fell within the clade comprising species, forming a coherent cluster with ATCC 23178 and NN015860, with which it shared 16S rRNA gene sequence similarities of 98.1 and 98.2 %, respectively. Sequence similarities between strain M-M6 and the type strains of other recognized species were in the range 92.4–93.8 %. Strain M-M6 contained MK-6 as the predominant menaquinone and iso-C, iso-C G, iso-C 3-OH, and Cω7 and/or iso-C 2-OH as the major fatty acids. The major polar lipids detected in strain M-M6 and the type strains of and were two unidentified lipids, one unidentified aminolipid and one unidentified aminophospholipid. The DNA G+C content of strain M-M6 was 35.4 mol%. Levels of DNA–DNA relatedness between strain M-M6 and JCM 8516 and JCM 21778 were 33 and 35 %, respectively. Differential phenotypic properties and phylogenetic and genetic distinctiveness distinguished strain M-M6 from all recognized species. On the basis of the data presented, strain M-M6 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M-M6 ( = KCTC 23498 = CCUG 60801).

Funding
This study was supported by the:
  • 21C Frontier Program of Microbial Genomics and Applications (Award 11-2008-00-002-00)
  • Program for Collection, Management and Utilization of Biological Resources (Award M10867010003)
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2012-06-01
2021-10-27
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