Algiphilus aromaticivorans gen. nov., sp. nov., an aromatic hydrocarbon-degrading bacterium isolated from a culture of the marine dinoflagellate Lingulodinium polyedrum, and proposal of Algiphilaceae fam. nov.
A strictly aerobic, halotolerant, Gram-stain-negative, rod-shaped bacterium, designated strain DG1253T, was isolated from a laboratory culture of the marine dinoflagellate Lingulodinium polyedrum (CCAP 1121/2). The strain was able to degrade two- and three-ring polycyclic aromatic hydrocarbons. It exhibited a narrow nutritional spectrum, preferring to utilize aliphatic and aromatic hydrocarbon compounds and small organic acids. Cells produced surface blebs and contained a single polar flagellum. The predominant isoprenoid quinone of strain DG1253T was Q-8. The fatty acid profile was dominated by C18 : 1ω7c. The mean DNA G+C content of strain DG1253T was 63.6±0.25 mol%. 16S rRNA gene sequence analysis placed this organism within the order Xanthomonadales of the class Gammaproteobacteria. Its closest relatives included representatives of the Hydrocarboniphaga–Nevskia–Sinobacter clade (≤89.9 % 16S rRNA gene sequence similarity) in the family Sinobacteraceae. On the basis of distinct phenotypic and genotypic characteristics, strain DG1253T is considered to represent a novel species of a new genus in the class Gammaproteobacteria, for which the name Algiphilus aromaticivorans gen. nov., sp. nov. is proposed. The type strain of the type species, Algiphilus aromaticivorans, is DG1253T ( = ATCC BAA-2243T = DSM 24793T). In addition, a new family, Algiphilaceae fam. nov., is proposed to accommodate the genus Algiphilus.
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Funding
This study was supported by the:
Marie Curie International Outgoing Fellowship
(Award PIOF-GA-2008-220129)
AmannR. I.,
BinderB. J.,
OlsonR. J.,
ChisholmS. W.,
DevereuxR.,
StahlD. A.1990; Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl Environ Microbiol 56:1919–1925[PubMed]
FelsensteinJ.2005phylip (Phylogeny Inference Package) version 3.67. Distributed by the author, Department of Genome Sciences, University of Washington, Seattle, USA.
FriedrichM. M.,
LipskiA.2008; Alkanibacter difficilis gen. nov., sp. nov. and Singularimonas variicoloris gen. nov., sp. nov., hexane-degrading bacteria isolated from a hexane-treated biofilter. Int J Syst Evol Microbiol 58:2324–2329 [View Article][PubMed]
GreenD. H.,
LlewellynL. E.,
NegriA. P.,
BlackburnS. I.,
BolchC. J. S.2004; Phylogenetic and functional diversity of the cultivable bacterial community associated with the paralytic shellfish poisoning dinoflagellate Gymnodinium catenatum
. FEMS Microbiol Ecol 47:345–357 [View Article][PubMed]
KowalewskaG.1999; Phytoplankton – the main factor responsible for transport of polynuclear aromatic hydrocarbons from water to sediments in the Southern Baltic ecosystem. ICES J Mar Sci 56:219–222 [View Article]
LaneD. J.1991; 16S/23S rRNA sequencing. In Nucleic Acid Sequencing Techniques in Bacterial Systematics pp. 115–175 Edited by
StackebrandtE.,
GoodfellowM.
New York: Wiley;
LiuR.,
ZhangY.,
DingR.,
LiD.,
GaoY.,
YangM.2009; Comparison of archaeal and bacterial community structures in heavily oil-contaminated and pristine soils. J Biosci Bioeng 108:400–407 [View Article][PubMed]
MaidakB. L.,
ColeJ. R.,
ParkerC. T.Jr,
GarrityG. M.,
LarsenN.,
LiB.,
LilburnT. G.,
McCaugheyM. J.,
OlsenG. J.
other authors 1999; A new version of the RDP (Ribosomal Database Project). Nucleic Acids Res 27:171–173 [View Article][PubMed]
MalletL.,
SardouJ.1964; Investigation on the presence of the BP-type PH in the plankton environment of the region of the Bay of Villefranche (Alpes-Maritimes region). CR Acad Sci (Paris) 258:5264–5267
MesbahM.,
PremachandranU.,
WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
MillardE. S.,
MinnsC. K.,
CharltonC. C.,
HalfonE.1993; Effect of primary productivity and vertical mixing on PCB dynamics in planktonic model ecosystems. Environ Toxicol Chem 12:931–946 [View Article]
MillerM. R.,
BridleA. R.,
NicholsP. D.,
CarterC. G.2008; Increased elongase and desaturase gene expression with stearidonic acid enriched diet does not enhance long-chain (n-3) content of seawater Atlantic salmon (Salmo salar L.). J Nutr 138:2179–2185 [View Article][PubMed]
PajeM. L.,
NeilanB. A.,
CouperwhiteI.1997; A Rhodococcus species that thrives on medium saturated with liquid benzene. Microbiology 143:2975–2981 [View Article][PubMed]
PruesseE.,
QuastC.,
KnittelK.,
FuchsB. M.,
LudwigW. G.,
PepliesJ.,
GlöcknerF. O.2007; SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Res 35:7188–7196 [View Article][PubMed]
SheuS.-Y.,
ChoN.-T.,
ArunA. B.,
ChenW.-M.2011; Proposal of Solimonasaquatica sp. nov., reclassification of Sinobacterflavus Zhou et al. 2008 as Solimonasflava comb. nov. and Singularimonasvariicoloris Friedrich and Lipski 2008 as Solimonasvariicoloris comb. nov. and emended descriptions of the genus Solimonas and its type species Solimonassoli
. Int J Syst Evol Microbiol 61:2284–2291 [View Article][PubMed]
SingletonD. R.,
RamirezL. G.,
AitkenM. D.2009; Characterization of a polycyclic aromatic hydrocarbon degradation gene cluster in a phenanthrene-degrading Acidovorax strain. Appl Environ Microbiol 75:2613–2620 [View Article][PubMed]
StamatakisA.2006; RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690 [View Article][PubMed]
StürmeyerH.,
OvermannJ.,
BabenzienH.-D.,
CypionkaH.1998; Ecophysiological and phylogenetic studies of Nevskia ramosa in pure culture. Appl Environ Microbiol 64:1890–1894[PubMed]
ThompsonJ. D.,
HigginsD. G.,
GibsonT. J.1994; clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
TindallB. J.1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
VolkmanJ. K.,
NicholsP. D.1991; Applications of thin layer chromatography-flame ionization detection to the analysis of lipids and pollutants in marine and environmental samples. J Planar Chromatogr 4:19–26
WangQ.,
GarrityG. M.,
TiedjeJ. M.,
ColeJ. R.2007; Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 73:5261–5267 [View Article][PubMed]
WilmotteA.,
Van der AuweraG.,
De WachterR.1993; Structure of the 16S ribosomal RNA of the thermophilic cyanobacterium Chlorogloeopsis HTF (‘Mastigocladus laminosus HTF’) strain PCC7518, and phylogenetic analysis. FEMS Microbiol Lett 317:96–100[CrossRef]
Algiphilus aromaticivorans gen. nov., sp. nov., an aromatic hydrocarbon-degrading bacterium isolated from a culture of the marine dinoflagellate Lingulodinium polyedrum, and proposal of Algiphilaceae fam. nov.