1887

Abstract

An actinomycete, designated XMU 198, was isolated from the rhizosphere soil of a pharmaceutical plant, Engl., collected in Xiamen City, China. 16S rRNA gene sequence analysis showed that the isolate exhibited highest sequence similarities with KACC 20148, Q41 and YIM 31975 (98.7, 98.4 and 98.2 %, respectively). The chemotaxonomic characteristics further supported the assignment of strain XMU 198 to the genus : -diaminopimelic acid in the cell-wall peptidoglycan; glucose and galactose with minor amounts of ribose as the whole-cell sugars; polar lipids comprising phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and unidentified phospholipids; a fatty acid profile characterized by the predominance of iso-C, iso-C and anteiso-C; and MK-9(H) as the main menaquinone. Gyrase subunit B gene () sequence analysis showed that the genetic distances between strain XMU 198 and all other members of the genus were greater than 0.014, the value used as the threshold for species delineation within this genus. A wide range of genotypic and phenotypic characteristics, as well as DNA–DNA relatedness between strain XMU 198 and DSM 17836 (41.18 %), Q41 (38.02 %) and DSM 15500 (50.58 %), distinguished the isolate from its closest phylogenetic neighbours. On the basis of the above data, a novel species of the genus , sp. nov., is proposed. The type strain is XMU 198 ( = DSM 24683 = NBRC 107914).

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2012-05-01
2019-12-08
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