1887

Abstract

During screening for 4-chlorophenol-degrading micro-organisms in activated sludge from industrial wastewater treatment, a Gram-positive, rod-shaped, aerobic bacterial strain, designated 2C6-43, was isolated and characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 2C6-43 belongs to the family , class , and is related most closely to CC-NMPT-T3 (98.8 % sequence similarity), 1A-C (97.6 %), TT02-04 (96.8 %), YIM 004 (96.6 %) and YIM 93316 (96.0 %). The G+C content of the genomic DNA of strain 2C6-43 was 66.2 mol%. Sugars from whole-cell hydrolysates found in strain 2C6-43 were rhamnose, ribose and galactose. The menaquinone MK-8(H) was detected as the predominant quinone. Polar lipid analysis of 2C6-43 revealed diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol and phosphatidylglycerol. An aromatic compound ring cleavage enzyme of catechol 1,2-dioxygenase was detected but catechol 2,3-dioxygenase was not detected in 2C6-43. A fatty acid profile with anteiso-C, iso-C and C as the major components supported the affiliation of strain 2C6-43 to the genus . However, the DNA–DNA relatedness between strain 2C6-43 and the type strains of five species of the genus ranged from 17 to 40 %, clearly showing that the isolate constitutes a new genospecies. Strain 2C6-43 could be clearly differentiated from its phylogenetic neighbours on the basis of some phenotypic, genotypic and chemotaxonomic features. Therefore, strain 2C6-43 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is 2C6-43 ( = KCTC 19801 = JCM 17459).

Funding
This study was supported by the:
  • , GAIA Project , (Award 173-091-006)
  • , Ministry of Environment, Republic of Korea
  • , WCU (World class University) Program of the National Research Foundation of Korea
  • , Ministry of Education, Science and Technology , (Award R33-10076)
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2012-07-01
2020-08-10
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