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Volume 62, Issue Pt_12

sp. nov., isolated from marine organisms Free

Abstract

Five strains (CAIM 1831, CAIM 1832, CAIM 1833, CAIM 1834 and CAIM 1836) were isolated from cultured sole () in two regions of Spain, two strains (CAIM 404 and CAIM 1294) from wild-caught spotted rose snapper () in Mexico, and one strain (CAIM 1835) from corals in Brazil. The 16S rRNA gene sequences of the novel isolates showed similarity to (98.2–98.3 %, GenBank accession no. AJ630103) and to a lesser degree to (97.2–97.3 %, X76336) and to (96.9–97.1 %, X74703). Multilocus sequence analysis clustered these strains closely together and clearly separated them from phylogenetically related species of the genus . Genomic fingerprinting by rep-PCR clustered the novel strains according to their geographical origin. Phenotypic analyses showed a large variation among the new strains, but many tests enabled them to be differentiated from other species of the genus . The mean Δ values between the strains analysed here and closely related type strains were above 6.79 °C. The values between the novel isolates were below 2.35 °C, well outside the limit suggested for the delineation of a bacterial species. The phenotypic and genotypic data presented here clearly place these new strains as a coherent group within the genus , for which we propose the name sp. nov. with CAIM 1831 ( = DSM 24595 = S277) as the type strain.

  • Published Online:
Funding
This study was supported by the:
  • CONABIO HA013 (Mexico)
  • Jacumar National Plan for Solea
  • Spanish Ministry of Agriculture and Fisheries
© 2012 IUMS
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2012-12-01
2024-04-18
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Vibrio alfacsensis sp. nov., isolated from marine organisms
Int J Syst Evol Microbiol 62, 2955 (2012); https://doi.org/10.1099/ijs.0.033191-0
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