1887

Abstract

Two strains of Gram-staining-negative, rod-shaped bacteria that were motile by gliding, N7d-4 and B4a-b5, were isolated during a study of culturable bacteria in soil cultivated with potatoes. These isolates grew at 15–37 °C and at pH 6.5–7.0. The major cellular fatty acids were summed feature 3 (iso-C 2-OH and/or Cω7), anteiso-C, iso-C, iso-C 3-OH and iso-Cω9. The major polar lipids were phosphatidyl--methylethanolamine and phosphatidylethanolamine. The strains contained d-18 : 0 and d-19 : 0 sphingosines. The DNA G+C contents of strains N7d-4 and B4a-b5 were 48.5 and 46.9 mol% (HPLC), respectively. A phylogenetic analysis based on 16S rRNA gene sequences showed that strains N7d-4 and B4a-b5 were affiliated with species in the family . Strains N7d-4 and B4a-b5 shared 99.9 % sequence similarity, and the most closely related type strains were TR6-06 (96.5 and 96.7 % sequence similarity, respectively), N7 (95.4 and 95.6 %) and LMG 22862 (94.0 and 94.4 %). Phenotypic data and phylogenetic inference clearly distinguished the two isolates from other species. Based on these data, the isolates are considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is N7d-4 ( = KCTC 22699  = DSM 22385).

Funding
This study was supported by the:
  • KRIBB research initiative program (Award KBM4111022)
  • National Research Foundation of Korea (NRF) (Award 2007-2001776)
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2013-04-01
2024-10-06
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