A novel anaerobic, moderately thermophilic bacterium (strain SGL43T) was isolated from Severo-Stavropolskoye underground gas storage reservoir (Russia). Cells of strain SGL43T were motile straight rods, 0.4 µm in diameter and 2.0–3.0 µm in length. The temperature range for growth was 28–65 °C, with optimum growth at 50 °C. The pH range for growth was 5.5–8.0, with optimum growth at pH 7.0–7.5. Growth of strain SGL43T was observed at NaCl concentrations of 0–4.0 % (w/v) with optimum growth at 1.0 % (w/v) NaCl. Substrates utilized by strain SGL43T included peptone, yeast extract, glucose, fructose, maltose, galactose, pyruvate and citrate. Products of glucose or citrate fermentation were acetate, hydrogen and CO2. Thiosulfate was reduced to sulfide. The DNA G+C content of strain SGL43T was 43.1 mol%. 16S rRNA gene sequence analysis revealed that strain SGL43T belongs to the order Thermoanaerobacterales (phylum ‘Firmicutes’). The closest relative of strain SGL43T was Thermoanaerobacterium saccharolyticum (86.2 % 16S rRNA gene sequence similarity with the type strain). Based on the data presented here, strain SGL43T is considered to represent a novel species of a new genus, for which the name Caloribacterium cisternae gen. nov., sp. nov. is proposed. The type strain of Caloribacterium cisternae, the type species of the genus, is SGL43T ( = DSM 23830T = VKM B-2670T).
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Funding
This study was supported by the:
Russian Foundation for Basic Research
(Award 09-04-00251-a)
Federal Agency of Science and Innovations
(Award 02.740.11.0077)
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