@article{mbs:/content/journal/ijsem/10.1099/ijs.0.033076-0, author = "Slobodkina, G. B. and Kolganova, T. V. and Kostrikina, N. A. and Bonch-Osmolovskaya, E. A. and Slobodkin, A. I.", title = "Caloribacterium cisternae gen. nov., sp. nov., an anaerobic thermophilic bacterium from an underground gas storage reservoir", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2012", volume = "62", number = "Pt_7", pages = "1543-1547", doi = "https://doi.org/10.1099/ijs.0.033076-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.033076-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A novel anaerobic, moderately thermophilic bacterium (strain SGL43T) was isolated from Severo-Stavropolskoye underground gas storage reservoir (Russia). Cells of strain SGL43T were motile straight rods, 0.4 µm in diameter and 2.0–3.0 µm in length. The temperature range for growth was 28–65 °C, with optimum growth at 50 °C. The pH range for growth was 5.5–8.0, with optimum growth at pH 7.0–7.5. Growth of strain SGL43T was observed at NaCl concentrations of 0–4.0 % (w/v) with optimum growth at 1.0 % (w/v) NaCl. Substrates utilized by strain SGL43T included peptone, yeast extract, glucose, fructose, maltose, galactose, pyruvate and citrate. Products of glucose or citrate fermentation were acetate, hydrogen and CO2. Thiosulfate was reduced to sulfide. The DNA G+C content of strain SGL43T was 43.1 mol%. 16S rRNA gene sequence analysis revealed that strain SGL43T belongs to the order Thermoanaerobacterales (phylum ‘ Firmicutes ’). The closest relative of strain SGL43T was Thermoanaerobacterium saccharolyticum (86.2 % 16S rRNA gene sequence similarity with the type strain). Based on the data presented here, strain SGL43T is considered to represent a novel species of a new genus, for which the name Caloribacterium cisternae gen. nov., sp. nov. is proposed. The type strain of Caloribacterium cisternae, the type species of the genus, is SGL43T ( = DSM 23830T = VKM B-2670T).", }