1887

Abstract

A bacterial strain, isolated from a sample of reef-building coral () collected off the coast of southern Taiwan, was characterized using a polyphasic taxonomic approach. The strain, designated sw-2, was Gram-staining-negative, aerobic, rod-shaped and motile, with subpolar flagella, and formed greyish pink colonies. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain sw-2 was most closely related to Och 239 (97.4 % sequence similarity) and Och 210 (96.4 %). The novel strain did not require NaCl for growth and exhibited optimal growth at 35–40 °C, at pH 7.5–8.0 and with 3–7 % (w/v) NaCl. It produced bacteriochlorophyll under aerobic conditions. Summed feature 8 (Cω7 and/or Cω6; 63.7 %) predominated in the cellular fatty acid profile. The novel strain’s major respiratory quinone was ubiquinone Q-10 and its genomic DNA G+C content was 68.8 mol%. The polar lipid profile consisted of a mixture of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol, phosphatidyldimethylethanolamine, sulfo-quinovosyl diacylglycerol and three uncharacterized phospholipids. The level of DNA–DNA relatedness between strain sw-2 and Och 239 was only 15.0 %. The results of physiological and biochemical tests allowed the clear phenotypic differentiation of the novel strain from all established species of the genus . Based on the genotypic, phenotypic and chemotaxonomic data, strain sw-2 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is sw-2 ( = LMG 25204 = BCRC 17966).

Funding
This study was supported by the:
  • , Academia Sinica , (Award 20082010)
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2012-06-01
2020-08-03
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