1887

Abstract

Three isolates of Gram-negative, rod-shaped, non-spore-forming bacteria were recovered from galls on chrysanthemum ( L.; Ch11, Ch12) and cherry plum ( var. ; AL9.3). All three isolates were able to cause crown galls on various plant species. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the three isolates were probably identical (100% sequence similarity) and closely related to (99.6 %), (98.7 %) and (98.1 %). Similar analysis based on the housekeeping genes , and also indicated that the novel isolates were identical and closely related to . The major cellular fatty acids of strain Ch11 were Cω7 (62.1 %), summed feature 2 (comprising C aldehyde, iso-C I and/or C 3-OH; 10.8 %), summed feature 3 (comprising Cω7 and/or iso-C 2-OH; 7.7 %) and C 3-OH (7.5 %). However, the DNA–DNA relatedness between Ch11 and LMG 156 was only 48 % and, unlike phylogenetically related established species, the novel isolates were able to utilize β-hydroxybutyric acid but not -fucose. Based on the phylogenetic and phenotypic evidence, the isolates are considered to represent a single novel species of the genus , for which the name sp. nov. is proposed; the type strain is Ch11 ( = LMG 26191 = CFBP 7420).

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2012-04-01
2019-10-18
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