1887

Abstract

A Gram-negative, oxidase-positive, catalase-negative, facultatively anaerobic, motile, curved rod-shaped bacterium, strain N384, was isolated from a marine sponge (; phylum Porifera) collected from a depth of 795 feet (242 m) off the west coast of Curaçao. On the basis of 16S rRNA gene sequencing, strain N384 was shown to belong to the genus , most closely related to LMG 20546 (98.8 % similarity), ATCC 27043 (98.5 %), ATCC 19109 (98.6 %) and DSM 21326 (98.2 %). The DNA G+C content of strain N384 was 41.6 mol%. An analysis of concatenated sequences of five genes (, , , and ; 4068 bp) demonstrated a clear separation between strain N384 and its closest neighbours and clustered strain N384 into the ‘Orientalis’ clade of vibrios. Phenotypically, the novel species belonged to the arginine dihydrolase-positive, lysine decarboxylase- and ornithine decarboxylase-negative (A+/L−/O−) cluster. The novel species was also differentiated on the basis of fatty acid composition, specifically that the proportions of iso-C, iso-C, C, iso-C, C, iso-C, Cω8 and C were significantly different from those found in and . The results of DNA–DNA hybridization, average nucleotide identity and physiological and biochemical tests further allowed differentiation of this strain from other described species of the genus . Collectively, these findings confirm that strain N384 represents a novel species, for which the name sp. nov. is proposed, with the type strain N384 ( = ATCC BAA-2122 = DSM 23640).

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2012-08-01
2021-04-11
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