1887

Abstract

A Gram-staining-positive, non-motile and non-spore-forming lactic acid bacterium, designated strain M2, was isolated from fermented brown algae () kimchi in South Korea. Cells of the isolate were facultatively anaerobic ovoids and showed catalase- and oxidase-negative reactions. Growth of strain M2 was observed at 4–35 °C and at pH 5.0–9.0. The G+C content of the genomic DNA was 42.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain M2 belonged to the genus and was most closely related to IH003, IH25, LMG 18811, DSM 5578, TMW2.694 and BFE 7000 with 98.9 %, 98.8 %, 98.8 %, 98.7 %, 98.5 % and 98.2 % sequence similarity, respectively. DNA–DNA hybridization values between strain M2 and KACC 12281, IH25, KACC 12256, KACC 13854, DSM 21144 and DSM 21478 were 13.8±3.2 %, 14.3±3.4 %, 9.9±1.0 %, 13.2±0.8 %, 22.4±4.9 % and 16.2±4.6 %, respectively, which allowed differentiation of strain M2 from the closely related species of the genus . On the basis of phenotypic and molecular properties, strain M2 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is M2 ( = KACC 15353  = JCM 17445).

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2012-05-01
2019-10-23
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