1887

Abstract

During an investigation of yeast biota in the rhizosphere of lentisk in Sardinian semi-arid areas, a strain was isolated that could not be assigned to any known species. The sequence of the D1/D2 domain of the large subunit rDNA gene revealed that the strain belonged to the genus and was phylogenetically related to a clade including , , and The novel isolate differed from members of this clade in its ability to assimilate -glucono-1,5-lactone and its very weak fermentation of glucose and sucrose; its assimilation profile was unique within the genus . Monosporal colonies were able to sporulate, indicating that the species is homothallic. It is proposed that the isolate represents a novel species, sp. nov., with LCF 1675 ( = CBS 11859) as type strain.

Funding
This study was supported by the:
  • , Italian Ministry of Agriculture (MiPAF)
  • , MARKERINBIO
  • , Italian Ministry of Education
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2012-03-01
2020-10-24
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References

  1. Barazani O., Golan-Goldhirsh A. 2009; Salt-driven interactions between Pistacia lentiscus and Salsola inermis . Environ Sci Pollut Res Int 16:855–861 [CrossRef][PubMed]
    [Google Scholar]
  2. Bolano A., Stinchi S., Preziosi R., Bistoni F., Allegrucci M., Baldelli F., Martini A., Cardinali G. 2001; Rapid methods to extract DNA and RNA from Cryptococcus neoformans . FEM Yeast Res 1:221–224[PubMed]
    [Google Scholar]
  3. Capriotti A. 1967; Saccharomyces servazzii n. sp. A new yeast from Finland soil. Ann Microbiol Enzimol 17:79–84
    [Google Scholar]
  4. Cardinali G., Bolano A., Martini A. 2001; A DNA extraction and purification method for several yeast genera. Ann Microbiol 51:121–130
    [Google Scholar]
  5. Chen R., Wei S. C., Jiang Y. M., Wang Q. M., Bai F. Y. 2010; Kazachstania taianensis sp. nov., a novel ascomycetous yeast species from orchard soil. Int J Syst Evol Microbiol 60:1473–1476 [CrossRef][PubMed]
    [Google Scholar]
  6. Drummond A. J., Ashton B., Cheung M., Heled J., Kearse M., Moir R., Stones-Havas S., Thierer T., Wilson A. 2009 Geneious v4.8. Available from http://www.geneious.com
  7. Edgar R. C. 2004; MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics 5:113 [CrossRef][PubMed]
    [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  9. Ferreira N., Belloch C., Querol A., Manzanares P., Vallez S., Santos A. 2010; Yeast microflora isolated from brazilian cassava roots: taxonomical classification based on molecular identification. Curr Microbiol 60:287–293 [CrossRef][PubMed]
    [Google Scholar]
  10. Flahou B., De Baere T., Chiers K., Pasmans F., Haesebrouck F., Ducatelle R. 2010; Gastric infection with Kazachstania heterogenica influences the outcome of a Helicobacter suis infection in Mongolian gerbils. Helicobacter 15:67–75 [CrossRef][PubMed]
    [Google Scholar]
  11. Hong S. G., Lee K. H., Kwak J., Bae K. S. 2006; Diversity of yeasts associated with Panax ginseng . J Microbiol 44:674–679[PubMed]
    [Google Scholar]
  12. Imanishi Y., Ueda-Nishimura K., Mikata K. 2007; Two new species of Kazachstania that form ascospores connected by a belt-like intersporal body: Kazachstania zonata and Kazachstania gamospora . FEMS Yeast Res 7:330–338 [CrossRef][PubMed]
    [Google Scholar]
  13. Inácio J., Pereira P., de Carvalho M., Fonseca A., Amaral-Collaço M. T., Spencer-Martins I. 2002; Estimation and diversity of phylloplane mycobiota on selected plants in a Mediterranean-type ecosystem in Portugal. Microb Ecol 44:344–353 [CrossRef][PubMed]
    [Google Scholar]
  14. Inácio J., Ludwig W., Spencer-Martins I., Fonseca A. 2010; Assessment of phylloplane yeasts on selected Mediterranean plants by FISH with group- and species-specific oligonucleotide probes. FEMS Microbiol Ecol 71:61–72 [CrossRef][PubMed]
    [Google Scholar]
  15. Kurtzman C. P., Fell J. W. (editors) 1998 The Yeasts: a Taxonomic Study, 4th edn. Amsterdam: Elsevier;
    [Google Scholar]
  16. Kurtzman C. P., Robnett C. J. 1998; Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73:331–371 [CrossRef][PubMed]
    [Google Scholar]
  17. Kurtzman C. P., Robnett C. J. 2003; Phylogenetic relationships among yeasts of the ‘Saccharomyces complex’ determined from multigene sequence analyses. FEMS Yeast Res 3:417–432 [CrossRef]
    [Google Scholar]
  18. Lee C. F., Liu C. H., Young S. S., Chang K. S. 2008; Kazachstania jiainicus sp. nov., an ascomycetous yeast species isolated from soil in Taiwan. FEMS Yeast Res 8:114–118 [CrossRef][PubMed]
    [Google Scholar]
  19. Lee C. F., Yao C. H., Liu Y. R., Young S. S., Chang K. S. 2009; Kazachstania wufongensis sp. nov., an ascosporogenous yeast isolated from soil in Taiwan. Antonie van Leeuwenhoek 95:335–341 [CrossRef][PubMed]
    [Google Scholar]
  20. Limtong S., Yongmanitchai W., Tun M. M., Kawasaki H., Seki T. 2007; Kazachstania siamensis sp. nov., an ascomycetous yeast species from forest soil in Thailand. Int J Syst Evol Microbiol 57:419–422 [CrossRef][PubMed]
    [Google Scholar]
  21. Lu H. Z., Cai Y., Wu Z. W., Jia J. H., Bai F. Y. 2004; Kazachstania aerobia sp. nov., an ascomycetous yeast species from aerobically deteriorating corn silage. Int J Syst Evol Microbiol 54:2431–2435 [CrossRef][PubMed]
    [Google Scholar]
  22. Mikata K., Ueda-Nishimura K., Hisatomi T. 2001; Three new species of Saccharomyces sensu lato van der Walt from Yaku Island in Japan: Saccharomyces naganishii sp. nov., Saccharomyces humaticus sp. nov. and Saccharomyces yakushimaensis sp. nov. Int J Syst Evol Microbiol 51:2189–2198 [CrossRef]
    [Google Scholar]
  23. Naumov G. I., Naumova E. S., Korhola M. 1995; Karyotyping relationships among species of Saccharomyces sensu lato: S. castellii, S. dairensis, S. unisporus, and S. servazzii . Syst Appl Microbiol 18:103–108 [CrossRef]
    [Google Scholar]
  24. Nisiotou A. A., Nychas G. J. 2008; Kazachstania hellenica sp. nov., a novel ascomycetous yeast from a Botrytis-affected grape must fermentation. Int J Syst Evol Microbiol 58:1263–1267 [CrossRef][PubMed]
    [Google Scholar]
  25. O’Donnell K. 1993; Fusarium and its near relatives. In The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Systematics pp. 225–233 Edited by Reynolds D. R., Taylor J. W. Wallingford: CAB International;
    [Google Scholar]
  26. Tamura K., Nei M. 1993; Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512–526[PubMed]
    [Google Scholar]
  27. Tamura K., Nei M., Kumar S. 2004; Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci U S A 101:11030–11035 [CrossRef][PubMed]
    [Google Scholar]
  28. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef][PubMed]
    [Google Scholar]
  29. Von Arx J. A., Rodrigues de Miranda L., Smith M. T., Yarrow D. 1977 The Genera of Yeasts and Yeast-Like Fungi vol. 14 Utrecht NL: CBS;
    [Google Scholar]
  30. Weber G., Spaaij F., van der Walt J. P. 1992; Kluyveromyces piceae sp. nov., a new yeast species isolated from the rhizosphere of Picea abies (L.) Karst. Antonie van Leeuwenhoek 62:239–244 [CrossRef][PubMed]
    [Google Scholar]
  31. Wu Z.-W., Bai F.-Y. 2005; Kazachstania aquatica sp. nov. and Kazachstania solicola sp. nov., novel ascomycetous yeast species. Int J Syst Evol Microbiol 55:2219–2224 [CrossRef][PubMed]
    [Google Scholar]
  32. Yarrow D. 1998; Methods for the isolation, maintenance and identification of yeasts. In The Yeasts: a Taxonomic Study, 4th edn. pp. 77–100 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier; [CrossRef]
    [Google Scholar]
  33. Zubkova R. D. 1971; Genus novum Saccharomycetacearum e Kazachstania . Bot Mater Gerb Inst Bot Akad Kazakh SSR 7:53–56 (in Russian)
    [Google Scholar]
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