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Abstract

A Gram-stain-negative, non-motile, ovoid or rod-shaped bacterial strain, designated L-6, was isolated from seawater of Baekdo harbour of the East Sea in Korea and its taxonomic position was investigated by using a polyphasic study. Strain L-6 grew optimally at 30 °C, at pH 7.5–8.0 and in the presence of 2 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain L-6 formed a cluster with the type strain of at a bootstrap resampling value of 100 %. Strain L-6 exhibited 16S rRNA gene sequence similarity values of 97.7 % to H 14 and of less than 96.2 % to the type strains of other species used in the phylogenetic analysis. The G+C content of the chromosomal DNA of strain L-6 was 60.9 mol%. The predominant ubiquinone found in strain L-6 and CIP 109922 was ubiquinone-10 (Q-10). The predominant fatty acid of strain L-6 and CIP 109922 was Cω7. The major polar lipids of strain L-6 were phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The mean level of DNA–DNA relatedness between strain L-6 and CIP 109922 was 17 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, demonstrated that strain L-6 is distinguishable from . On the basis of the data presented, strain L-6 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is L-6 ( = KCTC 23497  = CCUG 60799).

Funding
This study was supported by the:
  • , Program for Collection, Management and Utilization of Biological Resources , (Award M10867010003)
  • , 21C Frontier Program of Microbial Genomics and Applications , (Award 11-2008-00-002-00)
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2012-06-01
2021-03-08
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