1887

Abstract

A -like strain, 172205, was obtained from mangrove soil collected at Qinglan Harbour, Wenchang, Hainan, China. The strain was characterized by white aerial mycelium and long spore chains. Comparison of 16S rRNA gene sequences indicated that the strain represents a novel member of the genus , exhibiting highest levels of similarity (<98.29 %) to the type strains of members of the genus . However, DNA–DNA relatedness and phenotypic data readily distinguished strain 172205 from phylogenetically related type strains. The predominant menaquinones were MK-9(H) and MK-9(H). The major fatty acids were iso-C (10.31 %), anteiso-C (35.19 %), iso-C (20.24 %) and anteiso-C (10.05 %). The diagnostic phospholipid was phosphatidylethanolamine. The cell wall contained -diaminopimelic acid and meso-diaminopimelic acid and whole-cell hydrolysates contained ribose, galactose and glucose. The results of DNA–DNA hybridization, physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain 172205 from phylogenetically related type strains. Therefore, strain 172205 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 172205 ( = CGMCC 4.6825  = DSM 42035).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.032201-0
2012-03-01
2019-12-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/3/596.html?itemId=/content/journal/ijsem/10.1099/ijs.0.032201-0&mimeType=html&fmt=ahah

References

  1. Bentley S. D. , Chater K. F. , Cerdeño-Tárraga A. M. , Challis G. L. , Thomson N. R. , James K. D. , Harris D. E. , Quail M. A. , Kieser H. . & other authors ( 2002; ). Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). . Nature 417:, 141–147. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bérdy J. . ( 2005; ). Bioactive microbial metabolites. . J Antibiot (Tokyo) 58:, 1–26. [CrossRef] [PubMed]
    [Google Scholar]
  3. Chun J. , Youn H. D. , Yim Y.-I. , Lee H. , Kim M. Y. , Hah Y. C. , Kang S.-O. . ( 1997; ). Streptomyces seoulensis sp. nov.. Int J Syst Bacteriol 47:, 492–498. [CrossRef] [PubMed]
    [Google Scholar]
  4. Chun J. , Lee J. H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  5. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230.[PubMed] [CrossRef]
    [Google Scholar]
  6. Euzéby J. . ( 2010; ). List of prokaryotic names with standing in nomenclature. . http://www.bacterio.cict.fr/s/streptomycesa.html
  7. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  8. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  9. Gauze G. F. , Preobrazhenskaya T. P. , Sveshnikova M. A. , Terekhova L. P. , Maksimova T. S. . ( 1983; ). Opredelitel’ aktinomitsetov (Manual of Actinomycetes). Moscow:: Nauka;.
    [Google Scholar]
  10. Goodfellow M. , Kumar Y. , Labeda D. P. , Sembiring L. . ( 2007; ). The Streptomyces violaceusniger clade: a home for streptomycetes with rugose ornamented spores. . Antonie van Leeuwenhoek 92:, 173–199. [CrossRef] [PubMed]
    [Google Scholar]
  11. Hong K. , Gao A. H. , Xie Q. Y. , Gao H. , Zhuang L. , Lin H. P. , Yu H. P. , Li J. , Yao X. S. . & other authors ( 2009; ). Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China. . Mar Drugs 7:, 24–44. [CrossRef] [PubMed]
    [Google Scholar]
  12. Hopkins D. W. , Macnaughton S. J. , O’Donnell A. G. . ( 1991; ). A dispersion and differential centrifugation technique for representatively sampling microorganisms from soil. . Soil Biol Biochem 23:, 217–225. [CrossRef]
    [Google Scholar]
  13. Ikeda H. , Ishikawa J. , Hanamoto A. , Shinose M. , Kikuchi H. , Shiba T. , Sakaki Y. , Hattori M. , Ōmura S. . ( 2003; ). Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis . . Nat Biotechnol 21:, 526–531. [CrossRef] [PubMed]
    [Google Scholar]
  14. Iwai H. , Takahashi Y. . ( 1992; ). Selection of microbial sources of bioactive compounds. . In The Search for Bioactive Compounds from Microorganisms, pp. 281–302. Edited by Ōmura S. . . New York:: Springer;. [CrossRef]
    [Google Scholar]
  15. Jones K. L. . ( 1949; ). Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. . J Bacteriol 57:, 141–145.
    [Google Scholar]
  16. Kämpfer P. , Kroppenstedt R. M. , Dott W. . ( 1991; ). A numerical classification of the genera Streptomyces and Streptoverticillium using miniaturized physiological tests. . J Gen Microbiol 137:, 1831–1891.[CrossRef]
    [Google Scholar]
  17. Kelly K. L. . ( 1964; ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  18. Khan T. K. , Tamura T. , Takagi M. , Shin-ya K. . ( 2010; ). Streptomyces tateyamensis sp. nov., Streptomyces marinus sp. nov. and Streptomyces haliclonae sp. nov., isolated from the marine sponge Haliclona sp.. Int J Syst Evol Microbiol 60:, 2775–2779. [CrossRef] [PubMed]
    [Google Scholar]
  19. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  20. Labeda D. P. , Lechevalier M. P. , Testa R. T. . ( 1997; ). Streptomyces stramineus sp. nov., a new species of the verticillate streptomycetes. . Int J Syst Bacteriol 47:, 747–753. [CrossRef] [PubMed]
    [Google Scholar]
  21. Lechevalier M. P. , Lechevalier H. A. . ( 1980; ). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy, pp. 22–291. Edited by Dietz A. , Thayer D. W. . . Arlington, VA:: Society for Industrial Microbiology;.
    [Google Scholar]
  22. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  23. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. K. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  24. Oliver J. D. , Colwell R. R. . ( 1973; ). Extractable lipids of gram-negative marine bacteria: fatty-acid composition. . Int J Syst Bacteriol 23:, 442–458. [CrossRef]
    [Google Scholar]
  25. Ōmura S. , Ikeda H. , Ishikawa J. , Hanamoto A. , Takahashi C. , Shinose M. , Takahashi Y. , Horikawa H. , Nakazawa H. . & other authors ( 2001; ). Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites. . Proc Natl Acad Sci U S A 98:, 12215–12220. [CrossRef] [PubMed]
    [Google Scholar]
  26. Pospiech A. , Neumann B. . ( 1995; ). A versatile quick-prep of genomic DNA from gram-positive bacteria. . Trends Genet 11:, 217–218. [CrossRef] [PubMed]
    [Google Scholar]
  27. Saintpierre-Bonaccio D. , Amir H. , Pineau R. , Lemriss S. , Goodfellow M. . ( 2004; ). Streptomyces ferralitis sp. nov., a novel streptomycete isolated from a New-Caledonian ultramafic soil. . Int J Syst Evol Microbiol 54:, 2061–2065. [CrossRef] [PubMed]
    [Google Scholar]
  28. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  29. Sanglier J. J. , Haag H. , Huck T. A. , Fehr T. . ( 1996; ). Review of actinomycetes compounds 1990–1995. . Expert Opin Investig Drugs 5:, 207–223. [CrossRef]
    [Google Scholar]
  30. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, Technical Note 101. . Newark, DE:: Microbial ID;.
  31. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  32. Sui J. L. , Xu X. X. , Qu Z. , Lin H. P. , Ruan J. S. , Hong K. . ( 2011; ). Streptomyces sanyensis sp. nov., isolated from mangrove sediment. . Int J Syst Evol Microbiol 61:, 1632–1637. [CrossRef] [PubMed]
    [Google Scholar]
  33. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4, molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  34. Waksman S. A. , Henrici A. T. . ( 1943; ). The nomenclature and classification of the actinomycetes. . J Bacteriol 46:, 337–341.[PubMed]
    [Google Scholar]
  35. Wang C. , Xu X. X. , Qu Z. , Wang H. L. , Lin H. P. , Xie Q. Y. , Ruan J. S. , Hong K. . ( 2011; ). Micromonospora rhizosphaerae sp. nov., isolated from mangrove rhizosphere soil. . Int J Syst Evol Microbiol 61:, 320–324. [CrossRef] [PubMed]
    [Google Scholar]
  36. Ward A. C. , Goodfellow M. . ( 2004; ). Phylogeny and functionality: taxonomy as a roadmap to genes.. In Microbial Diversity and Bioprospecting, pp. 288–313. Edited by Bull A. T. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  37. Watve M. G. , Tickoo R. , Jog M. M. , Bhole B. D. . ( 2001; ). How many antibiotics are produced by the genus Streptomyces? . Arch Microbiol 176:, 386–390. [CrossRef] [PubMed]
    [Google Scholar]
  38. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  39. Wellington E. M. H. , Williams S. T. . ( 1978; ). Preservation of actinomycete inoculum in frozen glycerol. . Microbiol Lett 6:, 151–157.
    [Google Scholar]
  40. Wiese J. , Jiang Y. , Tang S. K. , Thiel V. , Schmaljohann R. , Xu L. H. , Jiang C. L. , Imhoff J. F. . ( 2008; ). A new member of the family Micromonosporaceae, Planosporangium flavigriseum gen. nov., sp. nov.. Int J Syst Evol Microbiol 58:, 1324–1331. [CrossRef] [PubMed]
    [Google Scholar]
  41. Williams S. T. , Goodfellow M. , Alderson G. , Wellington E. M. H. , Sneath P. H. A. , Sackin M. J. . ( 1983; ). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129:, 1743–1813.[PubMed]
    [Google Scholar]
  42. Xiao J. , Wang Y. , Luo Y. X. , Xie S. J. , Ruan J. S. , Xu J. . ( 2009; ). Streptomyces avicenniae sp. nov., a novel actinomycete isolated from the rhizosphere of the mangrove plant Avicennia mariana . . Int J Syst Evol Microbiol 59:, 2624–2628. [CrossRef] [PubMed]
    [Google Scholar]
  43. Xu J. , Wang Y. , Xu J. , Xie S. J. , Xiao J. , Ruan J. S. . ( 2009; ). Streptomyces xiamenisis sp. nov., isolated from mangrove sediment. . Int J Syst Evol Microbiol 59:, 472–476. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.032201-0
Loading
/content/journal/ijsem/10.1099/ijs.0.032201-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error